树苔浸膏中关键气味活性化合物

Identification of the Key Odorants in treemoss Extracts

摘要:

香气是评价浸膏品质的重要指标，通过研究浸膏中的香气成分可以为浸膏的应用和质量控制提供参考依据。利用固相微萃取（SPME）和溶剂辅助风味蒸发（SAFE）两种方法结合GC-MS对树苔浸膏中的挥发性香气成分进行分析。通过香气提取物稀释分析（AEDA）对树苔浸膏中的香气活性物质进行鉴定，结果表明利用SPME和SAFE两种前处理方法共鉴定出102种挥发性成分，香气活性成分为27种，其中香气稀释因子（flavor dilution factor，FD因子）较高（FD＞9）的为β-苔黑酚羧酸甲酯，戊酮酸乙酯，苔黑酚羧酸乙酯，赤星衣酸乙酯，壬酸乙酯，庚酸乙酯，苯乙酸乙酯。进一步的定量及气味活度值（OAV）计算再次确认关键气味活性化合物为β-苔黑酚羧酸甲酯，苔黑酚羧酸乙酯，庚酸乙酯。

Abstract:

Aroma is one of the important standards to evaluate extracts. Analysis flavor components will provide reference for extracts application and quality control standard. The volatile flavor components of treemoss extracts were analyzed by solid phase microextraction (SPME) and solvent-assisted flavor evaporation (SAFE) combined with gas chromatography-mass spectrometry (GC-MS) and the aroma active components were confirmed by gas chromatograph-olfactometer (GC-O) with aroma extract dilutions analysis (AEDA). It was shown that a total of 102 compounds in two treemoss extracts samples (S1 and S2) were identified. Among these, there were 27 active odorants in two treemoss extracts, and the odorants with FD > 9 were methyl β-orcinolcarboxylate, ethyl levulinate, ethyl orsellinate, ethyl hematommate, ethyl nonanoate, ethyl heptanoate and ethyl phenylacetate. Moreover, these active odorants were quantitated by internal standard method and its odor active values (OAV) were calculated; it was confirmed methyl β-orcinolcarboxylate, ethyl orsellinate and ethyl heptanoate were the key odorants of the treemoss extracts.