响应面法优化荷叶生物碱盐提取工艺及其纯化

Optimization of Extraction of Alkaloid Salts from Lotus Leaves by Response Surface Methodology and Purification

摘要:

在单因素实验的基础上，通过响应面法分析和优化提取方法，研究超声酸提法中酸的种类、酸的质量分数、超声时间、液固比（液体体积与固体质量的比值）、超声功率和温度对荷叶生物碱盐提取效果的影响以及D101型大孔树脂对提取物的纯化效果。结果表明：荷叶生物碱盐的最佳提取工艺条件为：加热温度60℃、超声功率500W、盐酸质量分数0.3%、超声时间41min、液固比27:1，此工艺条件下荷叶碱的得率为（4.12±0.05）mg/g。以体积分数为70%的乙醇作为洗脱液，纯化后的生物碱盐经分光光度法测得纯度为40.81%。

Abstract:

On the basis of single factor experiments, the effects of acid type, acid concentration, ultrasonic time, solid-liquid ratio, ultrasonic power and temperature on the extraction of alkaloid salts from lotus leaves were studied by response surface analysis and optimization of extraction methods. At the same time, the purification effect of D101 macroporous resin on the extract was explored. The results showed that the optimum technological conditions of lotus leaf alkaloid salts were as follows: heating temperature 60 C, ultrasonic power 500 W, concentration of hydrochloric acid 0.3%, ultrasonic time 41 min, liquid-solid ratio 27:1, and the yield of lotus leaf alkaloid was 4.12±0.05 mg/g. 70% ethanol was determined as elution concentration. The purity of purified alkaloid salts reached 40.81%.