蓝莓果渣花色苷提取工艺优化及其提取物的抗肿瘤活性

Optimization of extraction process of anthocyanins from blueberry pomace and its antitumor activity

摘要:

在单因素的基础上，通过正交实验优化蓝莓果渣花色苷在浸提、微波辅助和超声辅助提取法的工艺条件，并对比3种提取方式下花色苷组分及其提取物抗肿瘤活性。结果表明：微波辅助提取法获得花色苷含量优于浸提法和超声辅助提取法，花色苷提取条件为：微波功率300 W、微波时间60 s、料液比为1:40 g/mL，在此条件下，花色苷含量46.880.63 mg C3G/g，3种提取法均获得6种花色苷组分，分别为飞燕草素-3-葡萄糖苷、矢车菊素-3-葡萄糖苷、矢车菊素-3-芸香糖苷、牵牛花素-3-葡萄糖苷、锦葵素-3-半乳糖苷和芍药素-3.5-二己糖苷。3种提取方式获得的花色苷提取物对HepG2肝癌细胞和A549肺癌细胞生长和侵染均有抑制作用，且对A549肺癌细胞抑制效应更强

Abstract:

Three extraction methods in terms of organic solvent extraction (OSE), microwave -assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) extraction of anthocyanins from blueberry pomace (BP) were optimized by single factor test and orthogonal test, and anthocyanins components and anti-tumor activity were comparative analysised. The results showed that MAE is superior to OSE and UAE in obtaining anthocyanins content, and the optimum extracting parameters to achieve the highest anthocyanins contents of 46.880.63 mg C3G/g from BP was obtained under the microwave power of 300 W, extraction time of 60 s and solid-to-liquid ratio of 1:40 g/mL. Under the optimal combination of parameters, six anthocyanins components were obtained by the three extraction methods, namely, delphinidin-3-glucoside, cyanidin-3-glucoside, cyanidin-3-rutinoside, petunidin-3-glucoside, malvidin-3-galactoside and peonidin-3,5-two hexoside. The BP extracts obtained by three extraction methods inhibited the growth and the invasion ability of HepG2 hepatoma cells and A549 lung cancer cells, and the inhibition effect of A549 lung cancer cells was stronger.