葡萄糖功能化的无规聚合物与凝集素的相互作用（急）

Interaction of Glucose Functionalized Random Polymers with Lectins

摘要:

以6-O-乙烯基己二酸-D-吡喃型葡萄糖（OVNGlu）和温敏性的二聚乙二醇单甲醚甲基丙烯酸甲酯(DEGMA)为原料，通过酶促反应和可逆加成-断裂链转移(RAFT)聚合制备了温度敏感型的葡萄糖无规聚合物P(DEGMA-co-OVNGlu)。采用傅里叶红外光谱仪(FTIR)、核磁共振氢谱(1HNMR)、凝胶渗透色谱(GPC)、紫外-可见分光光度计(UV-vis)和动态光散射(DLS)等对聚合物的结构及与凝集素(Con A)之间的相互识别性能进行了研究。结果表明，P(DEGMA-co-OVNGlu)成功制备。紫外和动态光散射结果表明，含葡萄糖的无规聚合物对Con A具有一定的识别能力，并且随着缀合时间的延长，在20 min时，聚合物与凝集素的识别达到饱和，随后逐渐形成交联。

Abstract:

A temperature-sensitive glucose random polymer p (diethyleneglycol methacrylate) -co-p ( 6-O-vinyladipoyl-α-D-galactose) P(DEGMA-co-OVNGlu) was prepared by enzymatic reaction and reversible addition-fragmentation chain transfer (RAFT) polymerization using 6-O-vinyladipoyl-α-D-galactose (OVNGlu) and thermoresponsive diethyleneglycol methacrylate (DEGMA) as raw materials. The polymer structure and glycopolymer-lectin (Con A) mutual recognition were studied by means of Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (1H NMR), gel permeation chromatography (GPC), ultraviolet spectrophotometer (UV-Vis) and dynamic light scattering (DLS). The results showed that P(DEGMA-co-OVNGlu) was successfully prepared. Ultraviolet and dynamic light scattering results show that the random polymer containing glucose has certain recognition ability for Con A， with the extension of the conjugation time, the recognition of the glycopolymer and lectin reached saturation at 20 min, and then gradually formed cross-link.