野棉花化学成分及抗肿瘤活性研究

Study on chemical constituents and antitumor activity of Amenone vitifolia

摘要:

采用硅胶、Sephadex LH-20、制备液相等色谱方法对恩施地区银莲花属植物野棉花体积分数为70%的乙醇提取液进行分离纯化，运用NMR、MS等方法进行结构鉴定，并考察了化合物的抗肿瘤活性。结果显示：得到了10个化合物，包括木脂素类、黄酮类、皂苷类化合物。分别为络石苷元(Ⅰ)，牛蒡子苷元(Ⅱ)，绿原酸(Ⅲ)，紫云英苷(Ⅳ)，异槲皮素苷(Ⅴ)，银椴苷(Ⅵ)，络石苷(Ⅶ)，牛蒡子苷(Ⅷ)，水杨苷(Ⅸ)，薯蓣皂苷(Ⅹ)。其中化合物Ⅰ~Ⅱ、Ⅵ~Ⅶ、Ⅸ是首次从银莲花属中分离得到，化合物Ⅰ~Ⅶ、Ⅸ、Ⅹ是首次从野棉花中分离得到。MMT(四唑盐比色法）测试结果显示：化合物Ⅱ、Ⅷ、Ⅸ、Ⅹ在100 μmol/L浓度时对宫颈癌细胞HeLa的抑制率分别为10.5% , 7.0%, 0.5%及88.0%，其中化合物X的活性最好，高于阳性对照品顺铂的抑制率80.5%。

Abstract:

The 70% ethanol extract of Anemone vitifolia in Enshi area of HuBei province was isolated and purified by column chromatography on silica gel, Sephadex LH-20 and preparative liquid chromatography. Structures were proved by physicochemical properties, NMR and MS.The antitumor activity was tested by MTT assay. Results 10 compounds, including lignin, flavonoidsand saponins, were obtained from 70% ethanol extract of Anemone vitifolia , and were identified trachelogenin (Ⅰ), arctigenin (Ⅱ), chlorogenic acid (Ⅲ), astragalin (Ⅳ), isoquercitrin (Ⅴ), tiliroside (Ⅵ), tracheloside (Ⅶ), arctiin (Ⅷ), salicin (Ⅸ) and dioscin (Ⅹ). CompoundsⅠ~Ⅱ、Ⅵ~Ⅶ、Ⅸ were isolated from the genus Amenone for the first time; Compounds Ⅰ~Ⅶ、Ⅸ、Ⅹ were isolated  from Amenone vitifolia for the first time. Compounds Ⅱ、Ⅷ、Ⅸ、Ⅹ inhibited the proliferation of HeLa cells with inhibition rates of 10.5%, 7.0%, 0.5% and 88.0% at 100 μmol/L, and the inhibition rate of compound Ⅹ was higher than that of the positive control cisplatin by 80.5%.