新型功能性植物盐美白功效的体外评价

In vitro study on the whitening effect of new functional plant salts

摘要:

对松盐、梅盐与竹盐的美白功效进行了体外评价，分别研究其对体外酪氨酸酶的单酚酶和二酚酶活性的抑制作用，并以熊果苷为阳性对照，采用体外培养的B16黑素瘤细胞模型探究其对胞内酪氨酸酶活性、细胞增殖及迁移的影响。结果表明：植物盐元素组成丰富，且水溶液呈碱性，松盐、竹盐、梅盐对酪氨酸酶活性表现出极显著的浓度依赖的抑制作用（p＜0.01），当3种植物盐水溶液为50.0 g/L时，对酪氨酸酶单酚酶抑制率分别为97.1%、94.8%、48.2%，对二酚酶的抑制率均高于92.0%；当作用于B16细胞时，与原盐相比，5.0 g/L的植物盐对B16细胞生长抑制作用不明显，但会抑制其横向迁移；植物盐对B16细胞胞内酪氨酸酶活性抑制作用优于熊果苷，7.5 g/L的梅盐组的细胞内酪氨酸酶活性仅有8.76%，抑制效果好于松盐、竹盐。上述结果表明：植物盐可以抑制酪氨酸酶的活性，且对B16细胞无附加毒性，显示出作为一种无机酪氨酸酶抑制剂的潜力，在人体美白方面有较好的潜在应用价值。

Abstract:

The whitening effects of pine salt, plum salt and bamboo salt were evaluated in vitro, and their inhibitory effects on tyrosinase monophenolase and diphenolase activity were studied in vitro. The B16 melanoma cell model was used to explore effects of salts on intracellular tyrosinase activity, cell proliferation and migration and arbutin was used as a positive control. The results showed that plant salts are rich in elemental composition and the aqueous solutions are alkaline, and pine salt, bamboo salt and plum salt showed extremely significant concentration-dependent inhibition of tyrosinase activity (p<0.01). When the concentration of the three plant saline solution was 50.0 g/L, the inhibition rates of tyrosinase monophenolase were 94.8%, 94.8%, and 48.2%, respectively, and the inhibition rates of diphenolase were higher than 92.0%. When acting on B16 cells, compared with the original salt, the inhibitory effect of 5.0 g/L plants salt on the growth of B16 cells was not obvious, but it inhibited the lateral migration. The inhibitory effect of plant salt on the intracellular tyrosinase activity of B16 cells was better than that of arbutin, and the intracellular tyrosinase of 7.5 g/L plum salt group is only 8.76%, and the inhibition effect is better than that of pine salt and bamboo salt. The above results indicate that plant salt can inhibit the activity of tyrosinase and has no additional toxicity to B16 cells, showing its potential as an inorganic tyrosinase inhibitor, and has a good potential application value in human whitening.