酶/酸水解毛叶木姜子中键合态香味成分的比较

Comparison of enzymatic and acid hydrolysis of bound aroma compounds in Litsea mollis Hemsl.

摘要:

采用Amberlite XAD-2树脂吸附洗脱分离毛叶木姜子中的键合态香气物质，对分离得到的键合态香气物质在3种条件下进行水解释放研究，并采用气相色谱-质谱联用法(GC-MS)对水解后的键合态香气物质进行检测分析，结果表明：3种不同水解条件得到的键合态香气物质共有90种，主要为萜烯类和含氧萜类物质，其中香叶醇在两种酶水解条件下均含量最高，最高为果胶酶水解条件下含量达21798.79μg/L；共34种香气物质具有明显香气特征，两种酶水解下最显著香气特征为花香和甜香，酸水解条件下最显著香气特征为花香。酶水解与酸水解条件下得到的键合态香气物质存在明显的差异，在果胶酶水解条件下所检出的香气物质种类最多且含量高，因此更有利于毛叶木姜子键合态香气成分的释放。

Abstract:

Bound aroma compounds from Litsea mollis Hemsl. were separated by Amberlite XAD-2 column chromatography and analyzed by gas chromatography-mass spectrometry (GC-MS) at three different conditions. The purpose of this study is to definite the effects of different hydrolytic conditions on the bonding aroma compounds. The result shows that 90 bound aroma compounds were identified in three different hydrolysates totally, mainly including Oxygenated monoterpenes and terpenes. Geraniol was the most abundant compound under two enzymatic hydrolysis conditions. And the highest content was 21798.79μg/L under the condition of pectinase hydrolysis; A total of 34 aroma substances have obvious aroma characteristics. And the most significant aroma features are floral and sweet under the two enzymatic hydrolysis, and floral under the hydrolysis of acid. Significant differences were found in the composition of bound aroma compounds among the different hydrolysates. In contrast, the most kinds and high content of aroma compounds were detected under the condition of pectinase hydrolysis, which was more conducive to the release of the bonding aroma components of Litsea mollis Hemsl..