菜芙蓉多糖的表征与生物活性

Characterization and bioactivity of polysaccharide from Abelmoschus manihot (L.) Medic

摘要:

以菜芙蓉花醇提后的残留物为原料，通过水提醇沉法提取菜芙蓉多糖，测定多糖总糖含量及得率。经DEAE-52柱层析分离纯化菜芙蓉多糖〔Abelmoschus manihot (L.) Medic polysaccharide, AMP〕，确定AMP单糖组成，考察AMP的体外抗氧化能力，并对AMP保护质粒DNA及抑制肝癌细胞HepG2的增殖作用进行了考察。结果表明:菜芙蓉花多糖得率为13.47%，总糖含量为97.43%，AMP主要由鼠李糖、葡萄糖、阿拉伯糖、岩藻糖组成，其物质的量比为1.44∶5.44∶1.00∶5.37，体外清除自由基能力表现为O2－· > ABTS+· > ·OH > DPPH· > NO·，8 g/L AMP抑制H2O2诱导的DNA氧化损伤效果最好，在0.125~5.000 g/L范围内，随着AMP质量浓度的增加HepG2细胞的增殖抑制率逐渐增大，说明AMP具有抗肿瘤活性。

Abstract:

The polysaccharide was extracted from the ethanol-extracted debris of Abelmoschus manihot (L.) Medic flower by water extraction and alcohol precipitation method. The total sugar content and extraction rate were determined. The Abelmoschus manihot (L.) Medic polysaccharide (AMP) was obtained by purification through DEAE-52 column chromatography and monosaccharide composition of AMP were determined. The bioactivities including antioxidant activity in vitro, DNA protecting activity and hepatic cancer cell HepG2 growth inhibitory effect of AMP were investigated. The results showed that the extraction rate reached 13.47% and the content of total sugar was 97.43%. AMP was mainly composed of rhamnose, glucose, arabinose and fucose at a molar ratio of 1.44:5.44:1.00:5.37. The order of radicals scavenging capacities of AMP was O2－· > ABTS+· > ·OH > DPPH· > NO· , which suggested AMP had certain antioxidant activity in vitro. 8 g/L AMP had the best inhibition effect on DNA oxidative damage induced by H2O2 . The inhibitory ratio of HepG2 cell increased with the increase of AMP concentration ranging from 0.125 to 5.000 g/L, indicating that AMP had potential antitumor activity.