固体脂质对辅酶Q10脂质纳米囊的稳定性及透皮性能的影响

Effects of solid lipids on the stability and transdermal delivery of CoQ10-Nanosolve

摘要:

为了提高辅酶Q10（CoQ10）的透皮吸收效果，以辛酸癸酸甘油三酯（GTCC）为液体脂质，鲸蜡醇棕榈酸酯（CP）为固体脂质，制备得到了不同浓度固体脂质的CoQ10-脂质纳米囊，并对其稳定性及透皮性能进行了研究。结果表明，固体脂质的添加未对CoQ10-脂质纳米囊的粒径及储藏稳定性产生影响，粒径均为52nm左右，储藏180d后其粒径及外观均无明显变化。FT-IR结果表明，CoQ10与脂质纳米囊之间未发生化学反应且能被很好的包埋在脂质纳米囊中。通过闭合效应、体外透皮实验及激光共聚焦的观察发现，当CP质量浓度增加至70%和95%（以固液总脂质为基准，下同）时，CoQ10-脂质纳米囊能显著减少皮肤水分的蒸发，并更好的促进CoQ10的透皮吸收，使表皮层和真皮层的荧光强度明显增强。

Abstract:

In order to improve the transdermal delivery of CoQ10 (CoQ10), CoQ10-Nanosolve with different concentrations of solid lipids were prepared using caprylic caprate triglyceride (GTCC) as liquid lipid and cetyl palmitate (CP) as solid lipid. Its stability and transdermal properties were studied. The results showed that the addition of solid lipids had no effect on the particle size and storage stability of CoQ10-Nanosolve. The particle size of CoQ10-Nanosolve with different concentrations of solid lipids were about 52nm, and there was no significant change in particle size and appearance after 180 days. FT-IR results showed that there was no chemical reaction between CoQ10 and Nanosolve and it could be encapsulated in Nanosolve well. From the occlusive effects, in vitro transdermal experiments, and laser confocal microscopy, it was found that when the mass concentrations of CP increased to 70% and 95%, CoQ10-Nanosolve could significantly reduce the evaporation of water from the skin and better promote the transdermal absorption of CoQ10，and the fluorescence intensity of the epidermal layer and the dermal layer was significantly enhanced.