采用液液微萃取结合GC-MS分析不同层酒醅原酒的挥发性成分

Analysis of Volatile Components of Base Distillates Produced by Different Layers of Jiupei by Liquid-Liquid Microextraction Combined with GC-MS

摘要:

采用液液微萃取（Liquid-liquid microextraction，LLME）结合气相色谱/质谱联用仪（GC-MS）对古井贡酒同一窖池5种不同层酒醅所产原酒进行分析，选择其中25种经文献报道具有生物活性的化合物进行定量。化合物的加标回收率在82.22% ~ 105.83%之间，在5种原酒中总含量为A> C> E> B> D。采用主成分分析法对25种生物活性成分的定量结果进行主成分分析，结果表明：最上层酒醅所生产原酒A与其余酒样差异较大，体现在第二主成分上的区分；B、C、D、E四种原酒在第二主成分上表现出一定的相似性，在第一主成分上表现出差异性。该研究有助于了解不同层酒醅酒中的生物活性物质差异。

Abstract:

Liquid-liquid microextraction (LLME) combined with gas chromatography/mass spectrometry (GC-MS) was used to analyze the base distillates produced by five different layers of Jiupei in the same cellar of Gujinggongjiu ,and selected 25 kinds of reported biologically active compounds for quantification. The spiked recovery of the compounds were between 82.22% and 105.83%, and the order of total contents in the 5 base distillates was A> C> E> B> D. Principal component analysis was used to analyze the quantitative results of 25 kinds of biologically active compounds. The results showed that: A produced by the uppermost Jiupei differed greatly from the rest of the samples, and was distinguished on the second principal component; B, C, D, E had certain similarities on the second principal component and showed differences on the first principal component. This study was helpful for understanding the differences in bioactive substances among different base distillates.