一个衍生于苯并15-冠-5的双光子荧光钠离子探针

A Benzo-15-Crown-5-Derived Two-photon Fluorescence Probe for Sodium Ion

摘要:

钠离子在人体生理与病理上起着关键性的作用，特别是在神经传导、肌肉与心脏收缩、电解质平衡、阳离子运输和细胞容积等方面都发挥着至关重要的功能。因此，开发用于细胞中钠离子三维动态成像的双光子荧光探针显得尤为重要。以苯并15-冠-5和双氰基二苯代乙烯分别作为钠离子受体与双光子荧光团开发出探针DNa，对DNa的结构进行了表征鉴定。探针DNa具有诸如小的分子尺寸、大的双光子吸收截面（δTPA, 1054 GM）、极好的光稳定性、适宜的水溶性和优良的细胞渗透性等优点. DNa对Na 显示了优良的专一选择性，不受其它离子的干扰，对Na 的解离常数Kd ＝ 23 ? 2 mmol?L-1。DNa能用于细胞中Na 的三维成像，是一个性能优良的双光子荧光探针。

Abstract:

The sodium ion plays critical roles in many physiological and pathological processes such as nerve conduction and muscle and heart contraction; it modulates the electrolyte level, cation transport, and cell volume. Therefore, it is very important to develop two-photon fluorescence probes for three-dimensional dynamic imaging of sodium ions in cells. A novel two-photon fluorescence probe (DNa) for Na derived from dicyanostilbene as a two-photon fluorophore and benzo-15-crown-5 as a novel Na ligand was develped, and its structure was analyzed and identified. DNa possessed small molecule size, large two-photon absorption cross-section (1054 GM), noncytotoxic effect, excellent photostability, moderate water-solubility, and good cell-permeability. DNa showed excellent selectivity for Na and was not interfered by other ions. The dissociation constant of Na , kd = 23 ? 2 mmol L-1. DNa can be used in the three-dimensional imaging of Na in cells, so it is an excellent two-photon fluorescence probe.