Hf-ZnO酪氨酸酶生物传感器检测邻苯二酚

Tyrosinase biosensor based on hafnium doped zinc oxide (Hf-ZnO) to detect catechol

摘要:

以四氯化铪(HfCl4)和硝酸锌为原料，通过水热法制得Hf掺杂氧化锌纳米材料(Hf-ZnO)，并将Hf-ZnO、酪氨酸酶(Tyr)和壳聚糖(CS)修饰在玻碳电极(GCE)上,制得Tyr/Hf-ZnO/CS/GCE生物电极。利用FESEM、DLS、XRD和XPS对Hf-ZnO的结构和性能进行表征。采用循环伏安伏安法(CV)和计时电流法(IT)对Tyr/Hf-ZnO/CS/GCE电极进行电化学测试。结果表明，Tyr/Hf-ZnO/CS/GCE电极在pH 5和-50 mV的低电势下对邻苯二酚有最佳检测能力，对邻苯二酚检测的线性范围是0.5~47 μmol/L，灵敏度为195 μA/(mmol/L)，检测限是0.1215 μmol/L(S/N=3)。此外，该生物电极的稳定性和重复性好，可有效避免尿素、多巴胺、抗坏血酸等与邻苯二酚电活性相近物质的干扰。

Abstract:

Hafnium doped zinc oxide(Hf-ZnO) was prepared from tetrachloride hafnium (HfCl4) and zinc nitrate by hydrothermal method. Tyr/Hf-ZnO/CS/GCE was obtained by modifying tyrosinase (Tyr)、Hf-ZnO and chitosan (CS) onto Glassy carbon electrode (GCE). The structure and properties of the Hf-ZnO was characterized by FESEM, XRD, DLS and XPS. Electrochemical tests were conducted on the modified electrode Tyr/Hf-ZnO/CS/GCE by cyclic voltammetry (CV) and chronoamperometry (IT). The results showed that Tyr/Hf-ZnO/CS/GCE electrode had the best detection ability for catechol at a low potential of -50 mV and pH 5. The linear range of catechol detection was 0.5~47 μmol/L, the sensitivity was 195 μA/(mmol/L), and the detection limit was 0.1215 μmol/L(S/N=3). In addition, the biological electrode has good stability and repeatability, and can effectively avoid the electrical activities similar with catechol interference of urea, dopamine, and ascorbic acid.