红细胞膜封装负载姜黄素的金纳米粒子

Erythrocyte Membrane Encapsulates Gold Nanoparticles Loaded with Curcumin

摘要:

针对姜黄素在水中溶解性差、稳定性差、难吸收、代谢快等缺点，设计开发了红细胞膜仿生纳米载药系统（Au-Cur@RBC）。抗癌药物姜黄素通过疏水作用吸附到利于封装的小尺寸的金纳米颗粒表面，然后通过物理挤压的方式将载药纳米粒子包封到红细胞膜囊泡内。结果表明，制备的金纳米粒子尺寸均一度高，分散性良好，平均粒径为13.18 nm。金-姜黄素纳米颗粒中姜黄素的包封率为86.85%，载药率为20.25%。通过金纳米颗粒的负载，可显著提高姜黄素的稳定性，金-姜黄素纳米颗粒在水溶液中储存30天后仍保留了52%的姜黄素。同时，暗场显微镜结果表明，红细胞膜涂覆改善了制备的Au-Cur@RBC对暗场显微镜入射光线的折射与衍射能力，从而呈现更清晰的图像。上述结果表明所制备的仿生纳米颗粒展示出应用于癌症治疗与成像的潜力。

Abstract:

A red blood cell membrane bionic nano-drug loading system (Au-Cur@RBC) was designed and developed to improve the shortcomings of curcumin, such as poor stability in water, poor absorption and fast metabolism, etc. The anticancer drug curcumin is loaded on the surface of small-sized gold nanoparticles that are favorable for encapsulation through hydrophobic interaction, and then the drug-loaded nanoparticles are encapsulated in erythrocyte membrane vesicles by physical extrusion. The results show that the prepared gold nanoparticles have high uniformity, good dispersibility, and an average particle size of 13.18 nm. The encapsulation rate of curcumin in curcumin nanoparticles is 86.85%, and the drug loading rate is 20.25%. The loading of gold nano-particles can significantly improve the stability of curcumin, and gold-curcumin nanoparticles still retain 52% of curcumin after being stored in an aqueous solution for 30 days. At the same time, the results of dark field mi-croscopy show that the coating of red blood cells has improved the ability of Au-Cur@RBC prepared to refract and diffract the incident light of the dark field microscope, thereby presenting a clearer image. The above results indicate that the prepared bionic nanoparticles show potential for application in cancer treatment and imaging.