光甘草定和氧化白藜芦醇协同治疗黄褐斑的作用评价

Evaluation of synergistic treatment of chloasma with glabridin and oxyresveratrol

摘要:

采用体外酪氨酸酶抑制实验并结合响应曲面法优化了光甘草定和氧化白藜芦醇协同抑制酪氨酸酶配比；并将光甘草定和氧化白藜芦醇按较优活性配比制备成胶束溶液，中波紫外照射法UVB 法复制小鼠黄褐斑模型，给药治疗后，检测小鼠血清和皮肤组织中的超氧化物歧化酶及丙二醛。结果表明，光甘草定和氧化白藜芦醇的最佳配比为0.012∶0.020 （mol/L∶mol/L）；光甘草定胶束溶液、氧化白藜芦醇胶束溶液和光甘草定氧化白藜芦醇复配胶束溶液均可降低黄褐斑小鼠皮肤组织和血清中的脂质过氧化，提高超氧化物歧化酶活性，且与模型组相比，光甘草定氧化白藜芦醇复配组在血清中的超氧化物歧化酶活性升高了24.71 U/mL，丙二醛含量降低了7.90 U/mL；在皮肤组织中的超氧化物歧化酶活性升高了15.14 U/mgprot，丙二醛含量降低了1.60 U/mgprot，说明且光甘草定氧化白藜芦醇复配胶束溶液效果最佳。光甘草定和氧化白藜芦醇对黄褐斑的形成具有保护作用，并且两者具有协同作用，联用效果更好。

Abstract:

The synergistic inhibition ratio of glabridin and oxyresveratrol to tyrosinase was optimized by in vitro tyrosinase inhibition experiments and response surface method. The micelle solution of glabridin and oxyresveratrol was prepared according to the optimum activity ratio, and the model mice with chloasma was replicated by medium-wave ultraviolet irradiationUVB radiation. After the micelle solution treatment, the superoxide dismutase and malondialdehyde in the serum and skin tissues of the mice were detected. The results showed that the optimal ratio of glabridin and oxyresveratrol was predicted to be 0.012∶0.020 (mol/L∶mol/L). Glabridin micelle solution,oxyresveratrol micelle solution and glabridin oxyresveratrol compound micelle solution all reduced lipid peroxidation in skin tissue and serum of mice with chloasma, and increased superoxide dismutase activity, compared with model group, superoxide dismutase activity in serum of glabridin oxyresveratrol compound group increased by 24.71 U/mL, malondialdehyde content decreased by 7.90 U/mL; superoxide dismutase activity in skin tissue increased by 15.14 U/mgprot, malondialdehyde content decreased by 1.60 U/mgprot, show thatand glabridin oxyresveratrol compound micelle solution had the best effect. Glabridin and oxyresveratrol have protective effect on the formation of chloasma, and the two have synergistic effect which works better when combined.