中间苍白杆菌对含蜡原油除蜡降粘分析

Analysis on Wax Removal and Viscosity Reduction of Waxy Crude Oil With Ochrobactrum intermedium

摘要:

蜡质不仅使含蜡原油黏度升高，而且会析出积聚在管壁上降低管输效率。从原油污染淤泥中分离出一株产表面活性剂的烃降解菌F-1，经16S rDNA鉴定为中间苍白杆菌。与原油作用7 d 后，能将蜡含蜡量为15.2%的高含蜡原油处理为蜡含蜡量为9.1%的含蜡原油，原油蜡质量分数降低40.1%，原油黏度降低21%以上，细胞疏水性达28.1%。通过傅里叶红外光谱鉴定该菌产生的生物表面活性剂为脂肽类。添加该菌上清液到液体石蜡 可形成91.49 mm的排油圈，能够显著降低培养基表面张力，对液体石蜡乳化系数达到65%。

Abstract:

Waxy not only increases the viscosity of waxy crude oil, but also precipitates and accumulates on the pipe wall, reducing the efficiency of pipe transportation. A strain of surfactant producing hydrocarbon degrading bacteria F-1 was isolated from crude oil contaminated sludge and identified as Ochrobactrum intermedium by 16S rDNA. After the interaction with crude oil for 7 days, the high-wax crude oil with a wax content of 15.2% could be treated with a wax content of 9.1%, the crude oil wax mass fraction decreased by 40.1%, the crude oil viscosity decreased by more than 21%, and the cell surface hydrophobicity reached 28.1%. The biosurfactant produced by the bacteria was identified as lipopeptide by Fourier infrared spectroscopy. It can form a 91.49mm oil drainage ring, which can significantly reduce the surface tension of the medium and reach 65% emulsification coefficient of the liquid paraffin.