微流体技术制备聚合物纳米荧光探针

Microfluidics-based preparation of fluorescent polymer dots

摘要:

摘要：设计并制作了同轴共流毛细管微反应器，选用聚（9,9-二辛基芴-共-苯并噻二唑）（PFBT）作为聚合物，四氢呋喃（THF）作为溶剂，去离子水作为反溶剂，聚苯乙烯－马来酸酐共聚物（PSMA）作为稳定剂，结合纳米沉淀法制备聚合物纳米荧光探针，并使用动态光散射(DLS)进行表征。结果显示，在操作条件相同，通道尺寸分别为50 μm～500 μm时，通道尺寸越小，越有利于制备小粒径的纳米颗粒；当PFBT的质量浓度为1 μg/mL～50 μg/mL时，聚合物质量浓度越低，越有利于制备小尺寸的纳米颗粒；当通道尺寸为50 μm，PFBT质量浓度为5 μg/mL，去离子水与聚合物溶液的流量比达到40时，纳米颗粒的尺寸可下降到14 nm，实现了对纳米颗粒尺寸的精确控制并制备了尺寸更小的聚合物纳米颗粒。

Abstract:

Abstract: Coaxial co-flow capillary microreactors were designed and used to prepare fluorescent polymer dots (Pdots) using poly(9,9-dioctylfluorene-co-benzothiadiazole) (PFBT) as a polymer, tetrahydrofuran (THF) as a solvent, deionized water as an antisolvent, polystyrene - maleic anhydride copolymer (PSMA) as a stabilizer by a nanoprecipitation method. The size distributions of Pdots were measured by a dynamic light scattering (DLS) technique. The results showed that the sizes of polymer nanoparticles decreased as the microchannel diameters decreased in the range of 50 μm ~ 500 μm. For 1 μg/mL～50 μg/mL PFBT solutions, the sizes of polymer nanoparticles decreased with decreasing the mass concentration of the polymer. The diameters of polymer nanoparticles were reduced low to 14 nm using a 5 μg/mL PFBT solution at a flow ratio of deionized water to polymer solution of 40:1 in a microchannel with a diameter of 50 μm. The sizes and size distributions of nanoparticles were controlled accurately using the microfluidic technique presented here.