汉麻叶中大麻二酚的分离纯化及抗抑郁功能研究

Research on Separation, Purification and Antidepressant Function of Cannabidiol from Hemp Leaves

摘要:

目的：采用柱层析技术对汉麻叶中大麻二酚（Cannabidiol，CBD）进行分离纯化，并对其在抑郁症状方面进行应用探究。方法：汉麻叶经预处理后，通过超临界CO2萃取获得汉麻浸膏，树脂进行两步纯化获得CBD纯品。建立慢性不可预测的轻度应激模型小鼠，通过灌胃CBD来探究其抑郁行为及炎症因子的影响。结果：通过此方法进行分离纯化提取CBD纯度高达99.18%；通过动物实验得出，CBD剂量组均显著改善模型小鼠的抑郁行为（P<0.05）；模型组小鼠的皮层和海马组织中TNF-α、IL-1β和iNos mRNA表达量显著升高（P<0.05）；CBD剂量组的皮层和海马TNF-α、IL-1β和iNos mRNA表达量均显著降低（P<0.05；P<0.01）。结论：采用此方法制备获得CBD具有较高纯度，CBD能够有效改善小鼠的抑郁行为和抑制脑组织皮层和海马的炎症反应。

Abstract:

Purpose: To use column chromatography to separate and purify cannabidiol (Cannabidiol, CBD) from hemp leaves, and to explore its application in depressive symptoms. Method: After pretreatment of hemp leaves, supercritical CO2 extraction was used to obtain hemp extract, and the resin was purified in two steps to obtain pure CBD. The establishment of chronic unpredictable mild stress model mice, through intragastric administration of CBD to explore their depression behavior and the influence of inflammatory factors. Results: The purity of CBD extracted by this method was as high as 99.18%; through animal experiments, the CBD dose group significantly improved the depression behavior of model mice (P<0.05;); the expression of TNF-α, IL-1β and iNos mRNA in the cortex and hippocampus of the model group was significantly increased (P<0.05;); the expression of TNF-α, IL-1β and iNos mRNA in the cortex and hippocampus of the CBD dose group were significantly decreased (P<0.05). Conclusion: The CBD prepared by this method has high purity. CBD can effectively improve the depressive behavior of mice and inhibit the inflammatory response in the cerebral cortex and hippocampus.