富勒烯-二氧化锰多功能纳米复合物的合成及光动力学治疗性

Synthesis and photodynamic therapy of fullerene-manganese dioxide multifunctional nanocomposite

摘要:

以C60为原料，利用宾格尔环加成反应得到水溶性C60-COOH后，通过水热反应负载上MnO2纳米颗粒，最后利用氨基化聚乙二醇连接上肿瘤靶向分子叶酸，合成了一种多功能纳米复合物C60-Mn-PEG-FA。FTIR，UV-Vis，XPS和DLS确定了其结构组成和良好的水分散性。体外和细胞实验表明， C60-Mn-PEG-FA不仅可以实现低pH下的磁共振成像，还能改善乏氧；在40 ?g/mL的低剂量下，被100 mW/cm2强度的自然光照射5分钟就可以靶向杀伤82%的肿瘤细胞，而对正常细胞无损，为新型诊疗一体化制剂的设计提供了策略。

Abstract:

Herein, the water-soluble C60-COOH derivative was first obtained through Bingel cycloaddition reaction of C60, and then the MnO2 nanoparticles were loaded on it by hydrothermal reaction. Finally, the tumor targeting molecule folic acid was conjugated with the aminated polyethylene glycol to synthesize the multifunctional nanocomposite C60-Mn-PEG-FA. Its structural composition and good water dispersibility was confirmed by FTIR，UV-Vis, XPS and DLS. In vitro experiments, the results demonstrated that C60-Mn-PEG-FA could not only achieve magnetic resonance imaging (MRI) at low pH, but also improve hypoxia. At a low concentration of 40 ?g/mL and exposed to natural light of 100 mW/cm2 for 5 minutes, it could target and kill 82% tumor cells without damage to normal cells, providing a strategy for the design of novel theranostic agents.