红豆越橘花青素与金银花多酚协同抗氧化活性

Synergism Antioxidation of Lingonberries Anthocyanin and Lonicera japonica Polyphenols

摘要:

探讨红豆越橘花青素和金银花多酚协同抗氧化能力。测定单一组分和复配物（分别以红豆越橘花青素与金银花多酚质量比5:1、2:1、1:1、1:2、1:5复配）对DPPH自由基、ABTS自由基、羟基自由基、超氧阴离子的清除能力，对铜离子还原能力和总还原作用力，计算联合指数CI，分析各个比例的复配物是否具有协同抗氧化作用。除复配物（1:5）在清除DPPH自由基上表现为拮抗作用外，其它比例复配物在各项指标中均表现出协同效果。其中，复配物（2:1）对DPPH自由基清除率和对铜离子还原能力达50%时，CIDPPH=0.59±0.07，CI铜离子=0.35±0.03，表明二者以质量比2:1复配后对自由基的清除起到协同作用，具有协同抗氧化作用；复配物（1:1）对ABTS自由基和总还原作用力达50%时，CIABTS=0.58±0.04，CI总还原力=0.33±0.04，表明复配后，对自由基清除及金属离子还原能力具有协同抗氧化作用。红豆越橘花青素和金银花多酚复配具有很强抗氧化协同功效，可以平衡金银花的寒凉，缓解金银花用药量大，供不应求的现状，为天然绿色安全抗氧化保健品药品的开发提供理论基础。

Abstract:

Explore the synergistic antioxidant capacity of Lingonberries anthocyanin and Lonicera japonica polyphenols. Determination of single component and compound (respectively compounded with Lingonberries anthocyanin and Lonicera japonica polyphenol mass ratio 5:1, 2:1, 1:1, 1:2, 1:5) on DPPH free radical, ABTS free radical, hydroxyl radicals, superoxide anion scavenging ability, copper ion reducing ability and total reduction reaction, calculate the combination index CI, and analyze whether the compound in each proportion has a synergistic antioxidant effect. The results showed that, except the compound (1:5) showed antagonistic effect on scavenging DPPH free radical, the other proportion compound showed synergistic effect in each index. Among them, when the DPPH radical scavenging rate and copper ion reduction capacity of the complex (2:1) reached 50%, CIDPPH=0.59±0.07 and CI copper ion =0.35±0.03, indicating that the complex with mass ratio of 2:1 had a synergistic effect on the scavenging of free radicals and a synergistic antioxidant effect. When the effect of complex (1:1) on ABTS free radical and total reduction was 50%, CIABTS=0.58±0.04, and CI total reduction force =0.33±0.04, indicating that the antioxidant capacity was significantly improved after 1:1 combination. The experiment proved that the combination of Lingonberries anthocyanin and Lonicera japonica polyphenols have synergistic effect on the free radical scavenging and metal ion reduction ability. The results of the study show that the combination of Lingonberry anthocyanin and Lonicera japonica polyphenol has a strong antioxidant synergistic effect, which can balance the cold of honeysuckle, relieve the current situation of large amount of honeysuckle and short supply. It provides a theoretical basis for the utilization and development of natural green and safe antioxidant health products.