CMG-EGCG聚合物的合成及其抗氧化活性

Synthesis and antioxidant activity of CMG -EGCG copolymer

摘要:

采用1-(3-二甲基氨基丙基)碳二亚胺盐酸盐/4-二甲氨基吡啶化学偶联法将表没食子儿茶素没食子酸酯（EGCG）接枝到羧甲基酵母(1,3/1,6)-β-D-葡聚糖（CMG）的羧基上。通过FTIR、UV-Vis光谱和TGA对羧甲基酵母(1,3/1,6)-β-D-葡聚糖-EGCG（CMG-EGCG）的结构和热稳定性进行了表征，并分析了其抗氧化活性。Folin–Ciocalteu法测得CMG-EGCG中EGCG的含量为28.90 mg/g。结果表明，与CMG相比，CMG-EGCG具有更好的热稳定性和抗氧化活性，在质量浓度1.0×10-3 mg/L时，对DPPH自由基的清除率为78.85%，对ABTS自由基的清除率为93.00%，还原能力为1.15，可以应用于化妆品原料和食品工业。

Abstract:

Epigallocatechin gallate (EGCG) was successfully grafted onto the carboxyl group of carboxymethyl yeast (1,3/1,6)-β-D-glucan (CMG) by chemical coupling method of 1-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/4-dimethylaminopyridine (DMAP). The structure and thermal stability of carboxymethyl yeast (1,3/1,6)-β-D-glucan-EGCG (CMG-EGCG) were characterized by FTIR, UV-Vis spectra and TGA. The antioxidant activity of CMG-EGCG was evaluated. The EGCG content of CMG-EGCG measured by folin-Ciocalteu method was 28.90 mg/g. The results showed that compared with CMG, CMG-EGCG had better thermal stability and antioxidant activity. When mass concentration was 1.0×10-3 mg/L, CMG-EGCG showed 78.85% of inhibition of DPPH free radical, 93.00% of inhibition of ABTS free radical, and its reducing capacity was 1.15. Those results show that CMG-EGCG can be used in cosmetics raw materials and food industry.