草莓（Fragaria × ananassa）叶水提物对UVB致 皮肤损伤的保护作用

Protective effect of strawberry (Fragaria × Ananassa) leaf water extract on skin damage by UVB

摘要:

在草莓叶水提物（Strawberry leaf water extract，SLWE）制备基础上，测定SLWE成分，抗氧化活性、抑制非酶糖基化（NEG）能力，及SLWE对正常生长人皮肤成纤维细胞（HFF-1）细胞活率、透明质酸（HA）、人I型胶原蛋白（COL I）、羟脯氨酸（HYP）、基质金属蛋白酶1（MMP-1）、基质金属蛋白酶9（MMP-9）含量，评价SLWE延缓皮肤衰老功效。在中波紫外线（UVB）致HFF-1损伤模型基础上，测定SLWE对保护组活性氧（ROS）、丙二醛（MDA）、MMP-1和超氧化物歧化酶（SOD）的影响，探讨SLWE对UVB致HFF-1损伤保护机制。结果表明：SLWE富含Vc、槲皮素、绿原酸、没食子酸及儿茶素类物质，其中槲皮素含量12.86 mg/L，表儿茶素含量10.13 mg/L。SLWE自由基清除能力良好，清除不同自由基能力依次为O2-?＞DPPH＞ABTS＞亚铁离子螯和＞?OH；水溶液体积分数为1%的SLWE非酶糖基化抑制率为87.17%；体积分数大于3%的SLWE可提高HFF-1中HA、COL I和HYP含量（p＜0.05），体积分数为6%的SLWE可降低MMP-1含量（p＜0.01），说明SLWE具有提升皮肤含水量，增加胶原含量，抑制胶原降解，实现延缓衰老的功效；在SLWE对UVB致HFF-1损伤保护模型中，SLWE可有效降低因UVB刺激而产生的ROS，体积分数6%的SLWE可显著降MDA，抑制MMP-1表达，提高SOD活力，说明SLWE有助于缓解氧化应激造成的细胞损伤。

Abstract:

Based on the preparation of strawberry leaf water extract (SLWE), the composition, antioxidative activities and non-enzymatic glycosylation (NEG) inhibition rate of SLWE was explored. In addition, the effects of SLWE on the survival rate, hyaluronic acid (HA), collagen I (COL I) and hydroxyproline (HYP), matrix metalloproteinase 1 (MMP 1) and matrix metalloproteinase 9 (MMP 9) of normal growing human skin fibroblasts (HFF-1) were evaluated, in order to estimate the effect in delaying skin aging of SLWE. Through building UVB causes HFF-1 damage protection model, the effects of SLWE on reactive oxygen species (ROS), malondialdehyde (MDA), MMP-1 and superoxide dismutase (SOD) in the protection group were measured to explore the protective mechanism of SLWE on UVB-induced HFF-1 damage. The results showed that SLWE was rich in Vc, quercetin, chlorogenic acid, gallic acid and catechin, among which quercetin and epicatechin were 12.86 mg/L and 10.13 mg/L respectively. SLWE had good free radical scavenging ability, which was in order, O2-? > DPPH > ABTS > ferrous chelation > ?OH. The NEG inhibition rate of 1% (volume fraction) SLWE was 87.17%. More than 3% (volume fraction) SLWE could improve contents of HA, COL I and HYP (p < 0.05) in HFF-1, and 6% (volume fraction) SLWE could decrease the content of MMP 1 (p < 0.01), suggesting that SLWE was helpful to anti-aging through lifting the skin moisture, increasing the collagen content and inhibiting collagen degradation. In UVB causes HFF-1 damage protection model, SLWE could effectively ROS generated by UVB stimulation, 6% (volume fraction) SLWE could significantly reduce MDA, inhibit MMP-1 expression, and improve the activity of SOD, indicating that SLWE could help alleviate cell damage caused by oxidative stress.