等离子体引发微孔板接枝SMCC及蛋白吸附性能

Preparation of SMCC grafted micro-plates by plasma and the adsorption properties for protein

摘要:

用丙烯酰胺等离子体引发活化聚苯乙烯（PS）微孔板，接枝4-(N-马来酰亚胺基甲基)环己 烷-1-羧酸琥珀酰亚胺酯（SMCC）联接剂，制备了易于固定蛋白的PS-g-SMCC微孔板。利用FTIR、EDS、XPS、SEM、接触角测试仪和酶标仪对其表面组成、结构、形貌及蛋白吸附性能进行了表征。结果表明，PS微孔板表面成功接枝了丙烯酰胺与SMCC，当等离子体处理参数为500 W，300 s时，接枝丙烯酰胺的效果最好，PS-g-SMCC微孔板表面达到超亲水；当SMCC质量浓度为5 g/L 时，PS-g-SMCC微孔板对牛血清蛋白的吸附量最大，为903.08 ng/cm2，与未处理的PS微孔板相比，吸附量提高了2.93倍，有效提高了PS微孔板的检测灵敏性。

Abstract:

PS-g-SMCC micro-plates which are easy to immobilize proteins were prepared by initiating activated polystyrene (PS) micro-plates with acrylamide plasma and grafting succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate(SMCC) connector. The surface composition, structure, morphology and protein adsorption properties were characterized by FTIR, EDS, SEM, contact Angle tester and enzyme calibration. The results showed that acrylamide and SMCC were grafted successfully on the surface of PS micro-plates. When the discharge powers was 500 W and the processing time was 300 s, the grafting effect of acrylamide was the best, and the surface of the micro-plates showed excellent super-hydrophilic ability. When the SMCC concentration was 5 g/L, the maximum protein adsorption capacity of bovine serum protein was 903.08 ng/cm2. The protein adsorption capacity was 2.93 times higher than it of the untreated PS micro-plates, which effectively improved the sensitivity of PS micro-plates in ELISA detection.