多肽与多肽螯合铁叶面肥的制备及应用

Preparation and Application of Peptide Foliar Fertilizer and Peptide Chelated Iron Foliar Fertilizer

摘要:

以可食用明胶为原料，采用酸法制备了多肽叶面肥（Pep），再以Pep为螯合剂，硫酸亚铁为铁源，制备了多肽螯合铁叶面肥（Pep-Fe）。分别使用FTIR，SEM和TG对Pep和Pep-Fe的结构和形貌进行表征和观察，并进行小油菜种植实验。以多肽褐变、多肽氨基氮含量和多肽螯合铁螯合率为指标，优化出制备多肽与多肽螯合铁的反应条件。结果表明：温度70 ℃、反应时间7 h、明胶蛋白与水质量比为1：6、明胶蛋白与浓硫酸质量比为1：1.25条件下，制备的多肽不会发生褐变且氨基氮含量为125.49 mg/ml；温度50 ℃、反应时间1.5 h、pH4、多肽与硫酸亚铁质量比为2：1条件下，制备的多肽螯合铁螯合率为53.89%。

Abstract:

Peptide foliar fertilizer (Pep) was prepared from edible gelatin by acid method. Then Pep was used as a chelating agent and iron sulfate was used as a iron source to prepare peptide chelated iron foliar fertilizer (Pep-Fe). FTIR, SEM, and TG were used to characterize and observe the structure and morphology of Pep and Pep-Fe separately, and the planting experiment was carried out. The reaction conditions for the preparation of peptide and peptide chelated iron were optimized based on peptide browning, amino nitrogen content and iron chelating rate. The results showed that the optimum reaction conditions were as follows: temperature 70 ℃, reaction time 7 h, mass ratio of gelatin protein to water 1:6, mass ratio of gelatin protein to concentrated sulfuric acid 1:1.25. The prepared peptide did not turn brown and the amino nitrogen content was 125.49 mg/ ml. Under the conditions of temperature 50 ℃, reaction time 1.5 h, pH4, and the mass ratio of peptide to iron sulfate of 2:1, the chelating rate of iron was 53.89%.