仿生黏附性聚多巴胺丁香酚抑菌微胶囊的制备

Preparation of biomimetic adhesive polydopamine eugenol antibacterial microcapsules

摘要:

以丁香酚（EO）为芯材，多巴胺（DA）为壳材，通过乳液模板-界面聚合法成功制备出尺寸可控的聚多巴胺丁香酚（PDA@EO）微胶囊。通过FTIR、TG、UV-Vis、SEM和TEM对微胶囊的化学结构、形貌、粒径及性能进行表征和分析，结果表明，所制备的微胶囊呈规整球形，粒径在55～94 nm之间，丁香酚包封率为25.35%，包封量为0.6288 g/g，24 h时累积释放率达到68.39%。通过对比实验证明，PDA@EO微胶囊在不同材料表面均具有优异的黏附性能；作用于口腔感染部位常见细菌金黄色葡萄球菌和大肠杆菌24 h，PDA@EO微胶囊较游离丁香酚的抑菌活性分别提高了36.84%和35.52%；当微胶囊质量浓度达到2.0 g/L时，PDA@EO微胶囊对两种细菌的抑菌活性均达到99%以上。

Abstract:

Polydopamine eugenol microcapsules (PDA@EO) with controllable size were prepared by emulsion template interfacial polymerization using eugenol (EO) as the core material and dopamine (DA) as the shell material. The chemical structure, morphology, size and its distribution, and properties of the microcapsules were characterized by FTIR, TG, UV-Vis, SEM and TEM. The results showed that the microcapsules had regular spherical shape with size ranging from 55 to 94 nm. The encapsulation efficiency and the encapsulation capacity ware 25.35% and 0.6288 g/g, respectively. The cumulative release rate reached 68.39% after 24 h. The excellent adhesive property of PDA@EO microcapsules on different surfaces was verified by a comparative study. When treated with common bacteria in the site of oral infection Staphylococcus aureus (S·aure) and Escherichia coli (E·coli) for 24 hours, the growth of the cells was inhibited, PDA@EO Compared with free eugenol, the antibacterial activity of microcapsules increased by 36.84% and 35.52%, respectively; when the concentration of the microcapsule reached 2.0 g/L, the antibacterial activity of PDA@EO microcapsules against both bacteria was more than 99%.