基于静电纺丝制备的聚乙烯醇/高岭土止血膜

Polyvinyl alcohol (PVA)/kaolin hemostatic membranes based on electrospin technology

摘要:

采用静电纺丝技术将高分子聚合物聚乙烯醇/高岭土的水溶液固化，通过改变高岭土的含量，制得一系列止血膜。通过扫描电镜SEM与EDS图谱，分析纤维化止血膜与材料中高岭土颗粒的形貌和元素分布情况，结果表明,，高岭土能够有效分布于膜纤维中。凝血实验表明，由高岭土含量为2%、质量分数为10%的聚乙烯醇水溶液所制得的止血膜具有最佳的止血效果，其猪血的体外抗凝时间为(254 ± 2) s，止血SD大鼠肝损伤只需(167 ± 9) s，肝损伤失血量最小仅为(0.21 ± 0.05) g。进一步探究PK2止血膜的止血机理，发现其可有效激活内、外源性止血途径，对比空白对照组降低血浆凝固时间的比例分别为7.2%、4.7%，且在8 min左右即能生成较为紧密的凝血块。止血膜的低细胞毒性和低溶血率表明其具有较好的应用前景。

Abstract:

Solidified the polyvinyl alcohol water solution with different ratio of kaolin to fabricate hemostatic membranes by electrospinning. The SEM and EDS images showed that kaolin had been distributed on the membranes’ fibers. The hemostatic performance experiments showed that membranes made from10% PVA (mass ratio)/2% kaolin (mass ratio) solution performed best on hemostatic quality. It coagulate pig blood in vitro by 254 ± 2 s, and the blood loss and clotting time on SD rats’ hepatic injury experimentwere 167 ± 9 s and 0.21 ± 0.05 g, respectively. The further study on the hemostatic mechanisms, PK2 showed that it could decrease 7.2% and 4.7% clotting time on the APTT (activated partial thromboplastin time) and PT (prothrombin time) tests comparing with the controls, respectively, and it formed strong blood clots about 8 minutes. Combining with the low cytotoxicity and low hemolysis ration, it could be concluded that the PVA/kaolin electrospun membranes had high prospect on commercial application.