两相体系中生物转化肉桂醇生成天然2-苯乙醇

Biotransformation of cinnamyl alcohol into natural 2-phenylethanol in two-phase system

摘要:

利用肉桂内生菌鞘氨醇单胞菌Sphingomonas sp. Z45生物转化肉桂醇合成天然2-苯乙醇（2-PE），为了提高肉桂醇的转化率和产物2-PE的浓度，探究了在有机溶剂/水两相体系中进行生物转化的工艺条件。选择确定了油酸作为构建两相体系的有机相，在初始pH=7的10 mL发酵培养基中，加入1 mL种子液培养24 h后，同时加入底物肉桂醇3 g/L，油酸10 mL，在30℃、转速为200 r/min的摇床反应12 h，在此条件下肉桂醇转化率达88%，2-苯乙醇浓度为2.64 g/L。与单水相体系同等条件下（底物肉桂醇加入量3.0 g/L）的转化结果相比较，肉桂醇转化率提高了88%，2-苯乙醇的浓度提高了120%。

Abstract:

Cinnamyl alcohol can be biotranformed into natural 2-phenylethanol with the endophytic strain Sphingomonas sp.Z45. To improve the conversion yield of cinnamyl alcohol and the concentration of 2-PE, the biotransformation conditions in organic solvent-water biphase system were investigated. Oleic acid was selected as the organic phase of the biphase system. 1 mL seed liquid was inoculated in 10 mL fermentation medium and cultured for 24 h, and then 10 mL oleic acid and 3 g/L cinnamyl alcohol were added. The reactions were performed at 30 ℃ and 200 r/min for 12 h, the conversion of cinnamyl alcohol reached 88%, and the final concentration of 2-phenylethyl alcohol was 2.64 g/L. Compared with the result of the mono-aqueous system reaction under the same conditions（the amount of cinnamyl alcohol added was 3.0 g/L), the conversion yield of cinnamyl alcohol and the concentration of 2-phenylethanol were increased by 88% and 120% respectively.