含铁离子纳米络合物的制备及其诱导肿瘤细胞铁死亡作用

Preparation and effects on inducing tumor cells ferroptosis of iron containing nanocomplexs

摘要:

铁死亡是一种新发现的程序性细胞死亡方式，其过程与铁依赖的脂质过氧化代谢有关。以表没食子儿茶素没食子酸酯（EGCG）和Fe3+配合形成的FeⅢEGCG网络结构作为纳米载体，负载铁死亡诱导剂索拉菲尼（SRF），制备纳米络合物。通过对化合物进行粒径测定、Zeta电位测定及各物质含量比测定，对纳米颗粒进行表征。活性评价结果表明，含有SRF的纳米络合物对肿瘤细胞的生长抑制作用明显，肿瘤细胞存活率约为30%。同时发现纳米络合物存在多种形式的肿瘤细胞生长抑制相协同的效果，诱导细胞铁死亡确实是该纳米络合物的作用途径之一。

Abstract:

Ferroptosis is a newly discovered method of programmed cell death, the process of which is related to iron-dependent lipid peroxidation metabolism. In this paper, the FeⅢEGCG network structure formed by the coordination of epigallocatechin-3-gallate (EGCG) and Fe3 is used as the nanocarrier, and the ferroptosis inducer sorafenib (SRF) is loaded to prepare nanocomplexes. The nanocomplexes were characterized by particle size measurement, Zeta potential measurement and the content ratio measurement of each substance. The results of the activity evaluation showed that the nano drug containing SRF has obvious inhibitory effect on the growth of tumor cells, and the survival rate of tumor cells is about 30%. At the same time, it was found that the nanocomplex has a synergistic effect on tumor cell growth inhibition in various forms, and induction of cell ferroptosis is indeed one of the ways of action of the nanocomplex.