金银花多酚与红豆越橘乙醇提取物协同护肤

Synergistic efficacy of skin care of Lonicera japonica polyphenols and ethanol extract of Lingonberry

摘要:

以金银花多酚和红豆越橘乙醇提取物为原料，考察了金银花多酚与红豆越橘乙醇提取物单独及不同质量比例 〔m(金银花多酚)：m(红豆越橘乙醇提取物)=4:1、2:1、1:1、1:2、1:4〕联合应用在保湿、防晒、抗氧化、抑制黑色素和抗衰老方面的功效。采用称重法、稀释透过率法、2，2-联氮-二 (3-乙基-苯并噻唑-6-磺酸) 二铵盐 (ABTS)和1，1-二苯基-2-三硝基苯肼 (DPPH)自由基清除实验、酪氨酸酶抑制实验、弹性蛋白酶抑制实验、联合指数（CI）判断金银花多酚与红豆越橘乙醇提取物复配是否具有协同增效作用。结果显示，m(金银花多酚)：m(红豆越橘乙醇提取物)=1:2、1:4在保湿性能方面具有协同作用；m(金银花多酚)：m(红豆越橘乙醇提取物)=4:1、2:1和1:1在抗UVB性能方面具有协同作用；5个复配比例在清除ABTS自由基方面均有协同作用，CI值分别为0.79±0.036、0.83±0.041、0.85±0.026、0.96±0.03、0.98±0.027；5个复配比例在清除DPPH自由基方面均有协同作用，CI值分别为0.69±0.046、0.67±0.039、0.78±0.031、0.89±0.018、0.97±0.029；除m(金银花多酚)：m(红豆越橘乙醇提取物)=4:1外，其他复配比例在抑制酪氨酸酶活性方面均表现协同作用，CI值分别为0.71±0.054、0.68±0.047、0.76±0.033、0.81±0.06；m(金银花多酚)：m(红豆越橘乙醇提取物)=1:1、1:2和1:4复配比例在抑制弹性蛋白酶活性方面具有协同作用，CI值分别为0.84±0.053、0.88±0.042、0.86±0.041。金银花多酚与红豆越橘乙醇提取物联合应用在多个护肤功能上具有协同增效作用。结合其在5个护肤功能上的协同效果及表现能力综合分析，m(金银花多酚)：m(红豆越橘乙醇提取物)=1:1为最优复配比。

Abstract:

The functions of moisturizing, sunscreen, anti-oxidation, inhibiting melanin and anti-aging of single component and complexes of Lonicera japonica polyphenols and ethanol extract of lingonberry were determined by weighing, dilution transmittance method, the scavenging capacities of [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the tyrosinase inhibitory activity and elastase inhibitory activity, m(Lonicera japonica polyphenols): m(ethanol extract of lingonberry)=4:1, 2:1, 1:1, 1:2, 1:4. The Chou-Talalay combination index (CI) was used to analyze whether there were synergistic effects between the two. The results showed that m(Lonicera japonica polyphenols): m(ethanol extract of lingonberry)=1:2 and 1:4 showed synergistic effect on moisturizing; m(Lonicera japonica polyphenols): m(ethanol extract of lingonberry)=4:1, 2:1 and 1:1 exhibited synergistic effect on anti-UVB; all five complexes showed synergistic effects on scavenging ABTS free radicals, and the CI values were 0.79±0.036, 0.83±0.041, 0.85±0.026, 0.96±0.03, 0.98±0.027, respectively; all five complexes showed synergistic effects on scavenging DPPH free radicals, and the CI values were 0.69±0.046, 0.67±0.039, 0.78±0.031, 0.89±0.018, 0.97±0.029, respectively; m(Lonicera japonica polyphenols): m(ethanol extract of lingonberry)=4:1 showed antagonistic effect on inhibiting tyrosinase, while the other complexes exhibited synergistic effect, and the CI values were 0.71±0.054, 0.68±0.04, 0.76±0.033, 0.81±0.06, respectively; m(Lonicera japonica polyphenols): m(ethanol extract of lingonberry)=1:1, 1:2 and 1:4 exhibited synergistic effect on inhibiting elastase, and the CI values were 0.84±0.053, 0.88±0.042, 0.86±0.041, respectively. The results proved that the combination of Lonicera japonica polyphenols and ethanol extract of Lingonberry had synergistic effect on skin care. Combined with the performance on five skin care functions and synergistic effect, m(Lonicera japonica polyphenols): m(ethanol extract of lingonberry)=1:1 was the optimal complex for this study.