氧化石墨烯负载纳米银/聚乙烯醇型抗菌水凝胶制备与性能

Preparation and properties of graphene oxide silver-loaded polyvinyl alcohol antibacterial hydrogel

摘要:

以聚乙烯醇(PVA)、氧化石墨烯(GO)、硝酸银为原料，在不添加引发剂和交联剂的情况下，使用物理交联法（冷冻-解冻法）制得系列AgNPs质量分数不同的还原氧化石墨烯（rGO）负载纳米银/聚乙烯醇型抗菌水凝胶（rGO-AgNPs/PVA）（PGA）。通过FTIR、SEM对水凝胶的结构和形貌进行了表征，通过拉力实验和生物实验对其力学性能和生物性能进行了测试。结果表明，还原氧化石墨烯的加入增强了聚乙烯醇（PVA）水凝胶的机械强度，rGO-AgNPs/PVA抗菌水凝胶断裂伸长率相较于PVA水凝胶提高约60%，拉伸应变可达到125%。流变测试表明，PVA水凝胶的储能模量（G'）和损耗模量（G'）均低于rGO-AgNPs/PVA水凝胶；rGO与纳米银(AgNPs)协同抗菌，PGA-1、PGA-2、PGA-3、PGA-4、PGA-5对大肠杆菌和金黄色葡萄球菌的抑菌带宽度分别为0.5~4.5 mm和0.5~5.5 mm；SEM测试发现，相较于PVA水凝胶，rGO-AgNPs/PVA水凝胶的孔洞增多，rGO通过π-π作用形成网络结构，rGO-AgNPs/PVA水凝胶显示出多孔互联的微观结构。

Abstract:

Using polyvinyl alcohol (PVA), graphene oxide (GO) and silver nitrate as raw materials, a series of reduced graphene oxide (rGO) - supported nano silver / polyvinyl alcohol antibacterial hydrogels (rGO AgNPs / PVA) (PGA) with different mass fractions of AgNPs were prepared by physical crosslinking method (freezing thawing method) without adding initiator and crosslinker. The structure and morphology of the hydrogel were characterized by FTIR and SEM, and its mechanical and biological properties were tested by tensile test and biological experiment. The results showed that the addition of reduced graphene oxide enhanced the mechanical strength of polyvinyl alcohol (PVA) hydrogel, the elongation at break of rGO AgNPs / PVA antibacterial hydrogel was about 60% higher than that of PVA hydrogel, and the tensile strain could reach 125%. Rheological tests showed that the storage modulus (G') and loss modulus (G') of PVA hydrogel were lower than those of rGO AgNPs / PVA hydrogel; rGO and nano silver (AgNPS) are synergistic antibacterial. The antibacterial band widths of PGA-2, PGA-3, PGA-4 and PGA-5 against Escherichia coli and Staphylococcus aureus are 0.5~4.5 mm and 0.5~5.5 mm respectively; SEM test showed that compared with PVA hydrogel, the pores of rGO agnps/pva hydrogel increased, rGO formed network structure through π - π action, and rGO agnps/pva hydrogel showed porous interconnected microstructure.