基于咖啡酸的高灵敏性水合肼荧光探针的合成及应用

Synthesis and application of caffeic acid based fluorescent probe for highly sensitive detection of hydrazine hydrate

摘要:

以天然产物咖啡酸为原料，设计合成了一种可快速识别水合肼（N2H4）的咖啡酰基荧光探针ED。通过1HNMR、13CNMR和HRMS对其结构进行了表征。通过荧光光谱法研究发现探针ED对N2H4具有高选择和高灵敏性识别，检测过程中检测极限为0.31 μmol/L，最适pH范围为3~8，响应时间为48 s。通过MS和1HNMR证明探针ED对N2H4的荧光响应过程中结构中不饱和酮结构与N2H4反应后生成吡唑环。此外，探针ED与N2H4作用后溶液颜色从无色变为黄色，可实现N2H4的“裸眼检测”，并成功用于水样中N2H4的检测。 关键词：咖啡酸；荧光探针；水合肼；快速识别；检测试纸

Abstract:

A fluorescent probe (ED) was designed and synthesized using caffeic acid to detect N2H4 rapidly. Its structure was characterized by 1H NMR, 13C NMR, and HRMS. It was found that the probe ED had high selection and high sensitivity recognition of N2H4 by fluorescence spectroscopy, and the detection limit during the detection process was 0.31 μmol/L, the optimal pH value range was 3~8, and the response time was 48 s. MS and 1H NMR proved that the unsaturated ketone in the probe structure reacted with N2H4 to form pyrazole ring in the fluorescence response of probe to N2H4. In addition, after the probe ED interacts with N2H4, the color of the solution changes from colorless to yellow, which can realize the "naked eye detection" of N2H4. Probe ED was successfully used for the detection of N2H4 in actual water samples.