改性柑橘果胶-乳清蛋白稳定的精油Pickering乳液

Essential oils Loaded in Pickering emulsions Stabilized by Whey proteins- Modified citrus pectin

摘要:

以改性柑橘果胶和乳清蛋白为乳化剂，对月桂精油、百里香精油、牛至精油及佛手柑精油进行包埋，通过高压均质法制备了改性柑橘果胶-乳清蛋白复合纳米粒稳定的四种精油Pickering乳液。采用马尔文激光粒度仪、SEM、FTIR和哈克流变仪等对乳液进行了表征，并通过药敏实验评价了乳液的抑菌效果。结果表明，经过辐照处理后的改性果胶的乳化能力显著增强，提高至548.11 m2/g。红外光谱证明了聚合物的形成和精油的成功包封。乳液粒径大小在829~1147 nm之间，耐受离心且贮存77d后的稳定性均高于90%。流变学测试表明，四种乳液均属于假塑性流体。乳液对不同精油的包封率为5.81%~21.54%，负载率为0.81%~5.81%，且对包埋的精油具有控释效果。四种乳液对两种食品微生物（大肠杆菌和金黄色葡萄球菌）均具有显著抑制作用，抑菌圈范围为7.51~34.98 mm。

Abstract:

The Pickering emulsions of four essential oils stabilized by modified citrus and whey pectin composite nanoparticles were prepared by high-pressure homogenization method after irradiation modification of pectin. The emulsions were characterized by Malvern laser particle size analyzer, SEM, FTIR and Haake torque rheometer, and the emulsions and antibacterial effects were evaluated by drug sensitivity tests. The results showed that the emulsification ability of the irradiated modified pectin was significantly enhanced which the emulsion stability was increased to 548.11 m2/g. FITR spectra characterized the groups of the emulsions，corroborating the formation of aggregates and the successful encapsulation of essential oils. The particle size of the emulsions ranged from 829 nm to 1147 nm and were stable after centrifugation and stay more than 90% storage for 77 days. The emulsions encapsulated different essential oils in the range of 5.81% to 21.54% with loadings of 0.81% to 5.81%. The four emulsions showed significant inhibition of two food microorganisms (Escherichia coli and Staphylococcus aureus) with inhibition circles ranging from 7.51 to 34.98 mm. The modified citrus pectin-lactalbumin based Pickering emulsions were stable in structure and encapsulated essential oils with good slow release efficacy and antibacterial activity, which provides theoretical guidance for the establishment of emulsion systems and delivery of antimicrobial essential oils This provides a theoretical guide for the establishment of emulsion systems and the delivery of antimicrobial essential oils and a promising application pathway.