响应面法优化板栗苞中抑制痢疾杆菌活性成分的提取工艺

Optimization of extraction technology of active ingredients from chestnut burs for inhibiting Shigella dysenteriae byResponse Surface Methodology

摘要:

为提高板栗废弃物的资源利用率,本文以板栗苞为原料,探究其抑制痢疾杆菌活性成分的提取工艺。采用吸光光度法筛选板栗苞抑制痢疾杆菌活性成分的提取溶剂；以抑菌率为响应值,采用单因素实验结合响应面法优化板栗苞抑菌活性成分的提取工艺；采用二倍稀释法测定板栗苞各提取物的最低抑菌浓度(MIC),并采用吸光光度法研究其对细菌生长曲线的影响。结果表明,板栗苞的乙醇水提取物对痢疾杆菌的抑菌活性最好；板栗苞抑菌成分的最佳提取工艺为：乙醇浓度45%,料液比1:20(g:mL),提取温度50 ℃；在此最佳条件下,板栗苞提取物抑菌率为61.27%,MIC值为3.2 mg/mL；乙酸乙酯相提取物MIC值为0.4 mg/mL,抑菌率达85%,表明乙酸乙酯相萃取物有较强的抗菌活性,是一种极具开发潜力的天然抑菌剂。

Abstract:

In order to improve the resource utilization rate of chestnut waste, chestnut burs was used as raw material to explore the extraction process of the active ingredient for inhibiting Shigella dysenteriae. In this experiment, absorbance spectrophotometry was used to compare the antibacterial activity of different solvent extract of chestnut burs. The extraction process was optimized by single factor experiment combined with response surface methodology when antibacterial rate was used as response value and ethanol water was used as solvent. The minimum inhibitory concentration (MIC)of extracts from Chestnut burs were determined by double dilution method, and extracts on bacterial growth curve were compared by absorbance spectrophotometry. The results demonstrated that the ethanol water extract of Chestnut burs had the best antibacterial activity against Shigella dysenteriae. The optimal extraction process was as follow: 45% ethanol as solvent, the ratio of material to liquid was 1:20 (g:mL), extraction temperature 50 ℃; Under the optimal conditions, the antibacterial activity and MIC value of extract from Chestnut burs was 3.2mg /mL, at 0.5 mg/mL The antibacterial rate of extract was 61.27%. The MIC value of ethyl acetate phase extract was 0.4 mg/mL, and the antibacterial rate was 85%, indicating that ethyl acetate phase extract had strong antibacterial activity and was a natural antibacterial agent with great potential for development.