基于β-CD@AuNPs的荧光探针检测化妆品中的性激素

Fluorescent probe based on β-CD@AuNPs for detection of sex hormones in cosmetics

摘要:

本文研究了一种快速,高效地检测化妆品中性激素的荧光检测方法。以金纳米颗粒(AuNPs)作为此检测体系的荧光猝灭剂,罗丹明B(RB)作为荧光信号分子,β-环糊精(β-CD)作为特异性识别元件,基于荧光共振能量转移(FRET)原理实现了性激素的检测。在检测中RB先被β-CD捕获吸附在AuNPs的表面,RB和AuNPs之间的FRET效应猝灭了RB的荧光。激素分子的加入将RB从β-CD的疏水空腔中挤出,不再发生FRET,其荧光信号恢复。荧光恢复率与孕酮浓度呈良好的线性关系,范围约为0 μmol/L至25 μmol/L,检测限为0.15 μmol/L。该方法用于爽肤水中孕酮含量的测定,其回收率为95.3%~101.9%,相对标准偏差小于4.2%,且其对雌二醇、睾酮等性激素均有响应,表明此荧光探针对于化妆品中性激素的检测具有良好的应用价值。

Abstract:

This work develops a new method for rapid and efficient detection of sex hormones in cosmetics. Gold nanoparticles (AuNPs) with high dissipation coefficient was used as fluorescence quenchers in this detection system, rhodamine B(RB) was considered as fluorescence probe and β-cyclodextrin(β-CD) was provided as recognition element. Inclusion of RB into β-CD made fluorescence resonance energy transfer (FRET) occur through the donor (RB) and quencher (AuNPs) nearby. And then, the progesterone-induced release of RB from β-CD cavity, leads to the recovery of the fluorescence of RB. Thus the corresponding fluorescence recovery rate had a good linear relationship with concentration of progesterone in the range of 0 μmol/L-25 μmol/L,and the limit of detection is 0.15 μmol/L. The system was used for the determination of progesterone in toner, and the recoveries are from 95.3% to 101.9%, and the relative standard deviations were less than 4.2%. The fluorescence probe also response to other sex hormones such as estradiol and testosterone. It could be concluded that the system can be applied to the detection of sex hormones in cosmetics.