基于美拉德反应的羊毛无染料染色性能研究

Dye-free coloration of wool fabric by using Maillard reaction

摘要:

利用小分子还原糖-羊毛角蛋白的美拉德反应对羊毛进行无染料着色，分别通过SDS-PAGE凝胶电泳、分光光度法、DNS显色法以及染色织物得色量K/S的测定，研究糖与角蛋白的反应、糖的种类和反应条件对羊毛着色性能的影响。结果表明：还原糖与羊毛角蛋白发生反应形成了更大分子量的糖蛋白，反应液在540 nm处的吸光值发生明显变化，意味着还原糖与角蛋白发生美拉德反应形成了有色物质；相对于半乳糖和葡萄糖，木糖对羊毛着色具有更高的得色量；羊毛着色的适宜条件为反应温度90℃，反应pH =9；以过氧化氢对羊毛进行预处理，羊毛的得色量显著提高了23.0%；经美拉德反应着色的羊毛具有良好的耐洗色牢度、刷洗色牢度及耐干/湿摩擦色牢度，牢度级别分别达到4-5、5、5、5,但其耐光色牢度只达到2级。

Abstract:

Dye-free coloration of wool fabric was performed by using the Maillard reaction between reducing sugars and wool keratin. SDS-PAGE gel electrophoresis, spectrophotometry, DNS method and K/S value of the colored wool fabric were used to investigate the reaction between sugars and keratin, the effect of reducing sugar type and reaction conditions on the coloring property of wool fabric. The results showed that new glycoproteins with higher molecular weight were formed from the reaction between reducing sugar and wool keratin and the absorbance value at 540nm of the reaction solution changed obviously, which indicated that colorants were produced via the Maillard reaction between sugars and keratin. A stronger color yield of the colored fabric was obtained with xylose than that of galactose and glucose. The appropriate conditions for wool coloring were at 90℃ and pH=9. The colored yield of increased 23.0% after H2O2 pretreatment of wool fabric. The color fastness to washing and brushing and dry/wet rubbing fastness of the colored fabric via Maillard reaction were excellent with 4-5, 5, 5, 5 degree, respectively, but the color fastness to light is only 2 degree.