油茶花提取物的美白功效评价

Evaluation of whitening effect of Camellia oleifera flowers extract

摘要:

通过比较不同油茶副产品提取物的体外酪氨酸酶活性抑制作用,筛选出最佳抑制效果的油茶花提取物,并以曲酸作为阳性对照,采用体外培养的人A375黑素瘤细胞模型和斑马鱼胚胎模型探究其对体内外酪氨酸酶活性和黑色素合成的影响。结果表明,油茶花、油茶叶、油茶蒲和油茶粕提取物均表现出较好的酪氨酸酶活性抑制效果,其中油茶花效果最佳,其抑制IC50为0.49 mg/mL。与曲酸相比,油茶花提取物表现出极显著的胞内酪氨酸酶活性抑制作用和黑色素合成抑制作用(p<0.01),作用浓度为160 μg/mL时,胞内酪氨酸酶活性的抑制率达到34.30%,相对黑素含量减少了26.41%。当油茶花提取物暴露浓度为100 μg/mL时,其对斑马鱼体内酪氨酸酶抑制率达到32.47%,相对黑素含量降低至64.39%,作用效果与曲酸相当。油茶花提取物在体内外均表现出了较好的酪氨酸酶抑制效果,对细胞和斑马鱼黑色素生成具有显著抑制作用,且安全性高,是一种潜在的美白功能成分,在日用化妆品领域具有较高的应用前景。

Abstract:

By comparing the different Camellia oleifera extracts from by-products of the tyrosinase activity inhibition in vitro, and select the best inhibiting effect of Camellia oleifera flowers extract, and using the kojic acid as the positive control, using human A375 melanoma cells in vitro culture model and zebrafish embryo model to explore its influence of tyrosinase activity and melanin synthesis in vivo and in vitro. The results showed that Camellia oleifera flowers extract, Camellia oleifera leaves extract, Camellia oleifera shell extract and Camellia oleifera meal extract showed good inhibitory effects on tyrosinase activity, and Camellia oleifera flowers extract had the best inhibitory effect with an IC50 of 0.49 mg/mL. Compared with kojic acid, Camellia oleifera flowers extract showed significant inhibition of intracellular tyrosinase activity and melanin synthesis (P <0.01). When the concentration was 160 μg/mL, the inhibition rate of intracellular tyrosinase activity reached 34.30%, and the relative melanin content decreased by 26.41%. When the exposure concentration of Camellia oleifera flowers extract was 100μg/mL, the inhibition rate of tyrosinase in zebrafish reached 32.47%, and the relative melanin content was reduced to 64.39%. The effect was similar to kojic acid. Camellia oleifera flowers extract showed good tyrosinase inhibition in vitro and in vivo, and significantly inhibited melanin production in cells and zebrafish, with high safety. It is a potential whitening functional component, and has a high application prospect in the field of daily cosmetics.