GMA接枝海藻酸钠的光交联协同二价金属阳离子配位交联互穿网络凝胶的制备及释药性能评价

Preparation and evaluation of drug release properties of GMA-grafted sodium alginate photo-crosslinking with bivalent metal cationic coordination crosslinking interpenetrating network gels

摘要:

用甲基丙烯酸缩水甘油酯（GMA）对海藻酸钠（Na-Alg）进行接枝改性得到Alg-GMA，利用光引发自由基聚合协同二价阳离子（M2+，M代表Ca和Ba）配位交联作用，可调节海藻酸盐水凝胶的结构与性能。1HNMR结果表明，Alg-GMA中出现了与GMA相关的氢信号。在光引发剂Irgacure 2959存在下，Alg-GMA经波长365 nm紫外光照射90s即可快速形成Na-Alg-GMA水凝胶。SEM和压缩应力-应变结果显示，Ca2+和Ba2+具有调节GMA接枝光交联海藻酸盐凝胶结构与性能的作用，这可能与Ca2+的平面四方形构型dsp2轨道杂化方式和Ba2+的正八面体构型d2sp3轨道杂化方式有关。体外释放实验结果表明，Ca-Alg-GMA和Ba-Alg-GMA均具有肠道靶向释放药物能力。溶血率和MTT实验结果显示，Ca-Alg-GMA和Ba-Alg-GMA具有血液相容性好、细胞毒性低的特点。该研究拓展了基于海藻酸盐的新材料制备方法及其在生物医药领域中的应用空间，并提供了理论依据和实验支持。

Abstract:

In this paper, sodium alginate (Na-Alg) was grafted with glycidyl methacrylate (GMA), and the structure and properties of alginate hydrogel were adjusted by photoinitiated radical polymerization and bivalent cationic (M2+，M represents Ca or Ba) coordination crosslinking. The results of 1H NMR analysis showed that the hydrogen signal associated with GMA appeared in Alg-GMA. In the presence of photoinitiator Irgacure 2959, Alg-GMA can form hydrogel rapidly by irradiation of ultraviolet light with wavelength of 365nm in 90s. The results of SEM and compressive stress-strain showed that Ca2+ and Ba2+ could regulate the structure and properties of the photo-crosslinking alginate gel grafted by GMA, which may be related to the dsp2 hybridization mode of Ca2+ in the planar quadrate configuration and d2sp3 hybridization mode of Ba2+ in the regular octahedral configuration. The results of in vitro release experiments showed that both Ca-Alg-GMA and Ba-Alg-GMA had the ability of intestinal targeted drug release. The hemolysis rate and MTT test showed that Ca-Alg-GMA and Ba-Alg-GMA had good blood compatibility and low cytotoxicity. This study expands the preparation method of new materials based on alginate and its application space in the field of biomedicine, and provides theoretical basis and experimental support.