香芹酚/明胶/聚乙烯醇纤维膜的制备 及其抗氧化性能

Fabrication and antioxidant properties of carvacrol/gelatin/polyvinyl alcohol fibrous membranes

摘要:

为了实现香芹酚（CAR）的缓释，减缓食品的氧化变质进程，以可降解聚合物明胶（GEL）和聚乙烯醇（PVA）为基材，CAR为抗氧化剂，采用静电纺丝法制备香芹酚/明胶/聚乙烯醇复合纤维膜（CAR/GEL/PVA纤维膜）。通过SEM、FTIR、XRD对纤维膜的结构进行表征，并测定了纤维膜的抗氧化活性、缓释性能和橄榄油的过氧化值；考察了CAR添加量（以溶液中GEL的质量为基准）对纤维膜结构和性能的影响。结果表明：随着CAR添加量的增加，纺丝溶液的黏度增加，电导率下降，所制备的纤维膜形貌由直径较小且分布均匀转变为直径较大且分布不均匀，从而影响纤维膜的力学性能、抗氧化性能和缓释效果。Ritger-peppas和Weibull模型能较好地拟合缓释过程，其相关系数接近于1，释放规律遵循Fickian扩散机制，且当CAR添加量为5%时，纤维膜的缓释效果最佳。高CAR添加量的CAR/GEL/PVA纤维膜能在油脂类食物中缓慢释放，具有较强的抗氧化活性。在48 h时，CAR添加量为20%的纤维膜的DPPH自由基清除率为82.4%，比未添加CAR纤维膜提升了50.4%，可以有效降低橄榄油的过氧化值。

Abstract:

CAR/GEL/PVA active fibrous membranes were prepared by electrospinning using the biodegradable polymer gelatin (GEL) and polyvinyl alcohol (PVA) as substrates and carvacrol (CAR) as an antioxidant to achieve sustained release of CAR and slow down the oxidative deterioration of foods. The effects of CAR addition on the structure and performance of the fibrous membranes were analyzed by test and characterization of SEM, FTIR, XRD, antioxidant activity, sustained release performance, and peroxide value of olive oil. The results showed that with the increase of CAR addition (Based on the mass of GEL in the solution), the viscosity of the spinning solution increased and the electrical conductivity decreased, and the morphology of the prepared fiber membrane changed from a smaller diameter and uniform distribution to a larger diameter and uneven distribution, thus affecting mechanical properties, the antioxidant properties and sustained release effects of the fibrous membranes. The obtained fitting results of the Ritger-peppas and Weibull model were the best, with the correlation coefficient close to 1. The release mechanism followed Fickian"s law and the best sustained release effect was obtained with the addition of 5% CAR. CAR/GEL/PVA fibrous membranes with high CAR addition could release CAR slowly and had strong antioxidant activity in fatty foods. At the moment of 48h, DPPH radical scavenging rate of the fibrous membranes with 20% CAR addition was 82.4%, which was 50.4% higher than the one without CAR. Meanwhile, the increase of CAR addition could effectively reduce the peroxide value of olive oil.