比率型过氧化氢荧光探针的合成及成像应用

Synthesis and imaging of a ratiometric fluorescent probe for hydrogen peroxide

摘要:

采用6-甲氧基苯并噻唑-2-羟基喹啉作为双光子荧光团、硼酸酯作为过氧化氢（H2O2）识别基团，合成比率型检测H2O2的双光子荧光探针{6-甲氧基-2-[6-(4,4,5,5-四甲基-1,3,2-二氧硼杂环戊烷-2-基)喹啉-2-基]苯并[d]噻唑}（MQH2O2）。利用荧光光谱和双光子荧光光谱对探针进行H2O2响应能力的评估，结果显示探针具有良好的H2O2比率响应（30 min内比率信号增强约25.4倍、检出限低至38.6 nmol/L）和双光子性质（最大双光子荧光活性截面为150 GM）。通过双光子共聚焦成像完成了细胞和大脑组织成像，结果表明该探针能够实现脑卒中诱导细胞氧化应激的原位成像分析。

Abstract:

The synthesis of a two-photon fluorescence probe, {6-methoxy-2-(6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)quinolin-2-yl)benzo[d]thiazole} (MQH2O2), was achieved by incorporating 6-methoxybenzothiazol-2-hydroxyquinoline as the fluorescence moiety and borate ester as the H2O2 recognition element. Through the utilization of fluorescence spectroscopy and two-photon fluorescence spectroscopy, the ability of MQH2O2 to respond to H2O2 was evaluated. The results of the assessment reveal that the probe possesses a favorable H2O2 ratio response, with a ratio signal enhancement of roughly 25.4 times within 30 minutes and a detection limit as low as 38.6 nmol/L. Furthermore, the probe exhibits proficient two-photon properties, with a maximum two-photon fluorescence active cross-section of 150 GM. Finally, two-photon confocal imaging was utilized to conduct cell and brain tissue imaging, which demonstrated that the probe is capable of accomplishing in situ imaging analysis of oxidative stress caused by stroke.