二氧化碳还原用甲酸脱氢酶的研究进展

Advances in formate dehydrogenase for carbon dioxide reduction

摘要:

CO2的还原和资源化利用是缓解温室效应的重要手段。生物催化剂对反应和底物具有高选择性,因此被用于构建高效的CO2还原系统。其中甲酸脱氢酶(Formate dehydrogenase, FDH),特别是某些NAD+^依赖性/包含金属(W-或Mo-)辅因子的甲酸脱氢酶,能够可逆地将CO2还原成甲酸盐。本综述总结了最近发现的CO2还原用甲酸脱氢酶的还原活性及催化机制、酶工程改造、全细胞生物催化和基于CO2还原酶的应用进展,为CO2还原用生物催化剂将来的研究提供了较大的启示,为以丰富、廉价和可持续的CO2为原料,构建可行的CO2还原系统,以生产增值的燃料和化学品提供了理论基础。

Abstract:

CO2 reduction and resource utilization is an important means to alleviate Greenhouse Effect. Biocatalysts have high selectivity for reactions and substrates, so they are used to build efficient CO2 reduction systems. Among them, formate dehydrogenase (FDH) especially certain NAD⁺ -dependent/metal-containing (W- or Mo-) FDH are able to reversibly reduce CO2 to formate. This review summarizes the recent advances in reductive activity and catalytic mechanism of FDH for CO2 reduction, enzyme engineering, whole-cell biocatalysis and the application progress based on CO2 reductase. The innovative results summarized in this paper provide a great enlightenment and guidance for the future research of FDH for CO2 reduction, and provide a theoretical basis for the construction of feasible CO2 refining systems with abundant, cheap and sustainable CO2 as raw materials to produce value-added fuels and chemicals.