代谢组学探究刺梨提取物对高尿酸血症大鼠的作用

The effect of rosa roxburghii tratt extract on hyperuricemia rats by metabonomics

摘要:

采用氧嗪酸钾建立高尿酸血症（Hyperuricemia. HUA）大鼠模型，以别嘌醇为阳性药物对照，将刺梨提取物分为低、中、高剂量组。使用LC-MS技术对大鼠血清进行非靶向定性解析，运用多元统计方法进行大鼠血清数据分析并筛选出差异代谢物，通过MBRole 2.0通路分析功能进行代谢途径分析。结果显示，不同剂量的刺梨提取物均能降低HUA大鼠的尿酸水平，并在血清中鉴定出242种代谢物。在变量重要性投影（variable importance projection value, VIP>1）值、P（p-value）值<0.01以及差异倍数（fold change, FC≥2）的标准下，共筛选出44种显著差异代谢物，与差异代谢物相关的代谢途径共有58条。刺梨提取物降低尿酸可能与其对黄嘌呤氧化酶活性的抑制以及对血清脂质和氨基酸代谢的调节有关。这为刺梨在预防或治疗HUA方面提供了一定的理论依据。

Abstract:

Hyperuricemia. The HUA rat model was established with potassium oxonate. Allopurinol was used as positive drug control. Rosa roxburghii tratt extract was divided into low, medium, and high-dose groups. Non-targeted qualitative analysis of rat serum was performed using LC-MS technology, and multivariate statistical methods was used to analyze rat serum data and screen for differential metabolites. Metabolic pathway analysis was performed using the MBRole 2.0 pathway analysis function. The results showed that different rosa roxburghii tratt extract doses could reduce uric acid levels in HUA rats, and 242 metabolites were identified in the serum. Under the criteria of variable importance projection value (VIP>1), P (p-value) value<0.01, and fold change (FC ≥ 2), a total of 44 significantly different metabolites were screened, with a total of 58 metabolic pathways associated with different metabolites. The reduction of uric acid by rosa roxburghii tratt extract may be related to its inhibition of xanthine oxidase activity and regulation of serum lipid and amino acid metabolism. This provides a certain theoretical basis for the prevention or treatment of HUA in rosa roxburghii tratt.