静电纺丝紫苏醛/甲基-β-环糊精包合物纳米纤维的制备与表征

Preparation and characterization of electrospun antibacterial PA/MβCD inclusion complex nanofiber

摘要:

紫苏醛（PA）是一种从植物中提取的环保绿色天然活性成分，已被证明具有抗菌和抗氧化的特性。然而，PA的挥发性和水溶性较差限制了它的应用。本文以甲基-β-环糊精(MβCD)为主体分子，PA为客体分子，通过静电纺丝技术制备PA/MβCD包合物纳米纤维(PA/MβCD-IC-NF)。采用SEM对纤维的微观结构进行了表征。结果表明，PA/MβCD-IC-NF直径分布较均匀且纤维表面光滑，平均直径为（134±60） nm。运用1HNMR、XRD、FTIR和分子模拟等证明PA成功地被包裹在 MβCD空腔中，形成PA/MβCD包合物(PA/MβCD-IC)。此外，TGA和相溶解度研究结果表明，形成PA/MβCD-IC-NF后PA的热稳定性和水溶性都显著增强。抗氧化性实验和抑菌实验结果表明，PA/MβCD-IC-NF的抗氧化活性明显高于PA，对大肠杆菌与金黄色葡萄球菌的抑制率分别为96.2%±0.1%和94.0%±0.3%。

Abstract:

Perillaldehyde (PA) is an environment-friendly natural active ingredient extracted from plants that have been proven to possess antibacterial and antioxidant properties. However, the volatile nature and water-insolubility of PA limit its application. In this study, PA/MβCD inclusion nanofibers (PA/MβCD-IC-NF) were prepared by electrospinning technology. The microstructure of the fibers was characterized by SEM, and the results showed that the diameter distribution of PA/MβCD-IC-NF was relatively uniform and the surface was free of beads, with an average diameter of 134 ± 60 nm. _¹H NMR, XRD, FT-IR, and molecular simulation prove that PA was successfully encapsulated in the cyclodextrin cavity, forming PA/MβCD inclusion complex (PA/MβCD-IC). In addition, the results of TGA and phase solubility study displayed that the thermal stability and water solubility of PA were significantly enhanced after the formation of PA/MβCD-IC-NF. The antioxidant activity and antibacterial activity of the encapsulated nanofibers were evaluated and the results showed that the antioxidant activity of PA/MβCD-IC-NF was significantly better than that of PA, and the inhibitory rate against E. coli and S. aureus were 96.2±0.1% and 94.0±0.3%, respectively.