季铵盐化糖原衍生物/明胶复合水凝胶抗菌材料的制备及性能

Preparation and Properties of Quaternary Ammonium Glycogen Derivative-Gelatin Composite Hydrogel as Antibacterial Materials

摘要:

通过逐步修饰制备强正电性的糖原衍生物,第一步利用乙二胺对糖原进行氨基化修饰得到氨基化糖原衍生物(N-Gly),第二步利用2,3-环氧丙基三甲基氯化铵(季铵盐)对N-Gly进行部分季铵盐化修饰,得到季铵盐化糖原衍生物(QA-N-Gly)。然后以明胶为基材通过酰胺键交联制备季铵盐化糖原衍生物/明胶复合水凝胶(QA-N-Gly/Gel)。对糖原衍生物进行红外光谱(FTIR)、核磁共振波谱(1H-NMR)、粒径、Zeta电位以及微观形貌的考察,结果表明糖原的氨基化和季铵盐化修饰成功,QA-N-Gly为均匀分散的纳米粒子,粒径分布在100-200 nm,Zeta电位在90-100 mV,粒子带有明显的正电荷,具有抗菌性。对QA-N-Gly/Gel水凝胶进行理化性能和生物性能评价。结果表明糖原衍生物的加入可以提高明胶基水凝胶体系的机械强度和稳定性,QA-N-Gly/Gel的断裂应力为66.8 KPa,压缩模量为13.3 KPa。QA-N-Gly/Gel具有明显的抑菌作用,对金黄色葡萄球菌和大肠杆菌的抑菌率分别达到97%和50%。此外,QA-N-Gly/Gel无溶血性,细胞毒性低,NIH-3T3细胞的48 h存活率在80%以上。

Abstract:

Glycogen derivative with strong positive was synthesized by two steps. Firstly, the glycogen was modified by ethylenediamine to obtain the aminated glycogen derivative (N-Gly). Secondly, N-Gly was modified by 2, 3-epoxypropyl trimethyl ammonium chloride (GTA) to obtain the cationic quaternary ammonium glycogen derivative (QA-N-Gly). Then, quaternary ammonium glycogen derivative-gelatin composite hydrogel (QA-N-Gly/Gel) was prepared by amide central crosslinking. The chemical structure of glycogen derivative was characterized by FTIR and 1H-NMR. The particle size distribution, Zeta potential and morphology of glycogen derivative were also investigated. The results showed that glycogen was successfully aminated and quaternary ammonium modified. The QA-N-Gly were dispersed nanoparticles with a size distribution of 100-200 nm and a Zeta potential of 90-100 mV. QA-N-Gly exhibited antibacterial effect because of significant positive charge. The physicochemical and biological properties of QA-N-Gly/Gel hydrogel were evaluated. The results showed that the addition of glycogen derivatives could improve the mechanical strength and stability of gelatin-based hydrogel. The fracture stress of QA-N-Gly/Gel was 66.8 KPa, and the compression modulus was 13.3 KPa. QA-N-Gly/Gel showed obvious antibacterial effect on Staphylococcus aureus and Escherichia coli, and the inhibition rate reached 97% and 50%, respectively. In addition, QA-N-Gly/Gel showed no hemolysis and low cytotoxicity, the 48 h survival rate of NIH-3T3 cells was more than 80%.