无患子水提液的微生物发酵纯化及无患子乳酸杆菌发酵液抗痤疮功效

Microbial fermentation purification of sapindus mukorossi extract solution and anti-acne effect of Lactobacillus sapindus sapindus fermentation broth

摘要:

无患子（Sapindus mukorossi Gaertn）提取液常作为一种天然活性原料添加到洗护产品中，为推进无患子提取液在日化行业的应用，采用微生物发酵法对无患子水提液纯化，以耗糖量和皂苷纯度为指标，筛选出植物乳酸杆菌（Lactobacillus plantarum）作为发酵菌株，制得了无患子乳酸杆菌发酵液（SF）。从皮脂、炎症和致病菌的过度繁殖3个方面，研究了SF的抗痤疮功效。结果显示，质量浓度为20 μg/mL的SF对SZ95细胞脂质合成率降低了26.4%；对脂多糖（LPS）诱导的RAW 264.7细胞的一氧化氮（NO）、肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）和活性氧（ROS） 分别降低了52.3%、57.5%、56.2%和35.9% ；采用二倍稀释法测定了SF对痤疮丙酸杆菌、糠秕马拉色菌和金黄色葡萄球菌的最小抑菌质量浓度（MIC）别为3.125、12.5和6.25 g/L；人体功效评价结果显示，受试者面部痤疮情况显著改善。

Abstract:

Sapindus mukorossi Gaertn extracting solution as a natural active raw material to care products, in order to promote the application of Sapindus mukorossi Gaertn extracting solution in daily chemical industry, the water extraction was purified by microbial fermentation method. Lactobacillus plantarum was selected as the suitable fermentation strain based on sugar consumption and saponin purity, Sapindus mukorossi fermented liquid (SF) was prepared. The anti-acne effect of SF was studied from the three major causes of acne: sebum, inflammation and overgrowth of pathogenic bacteria. The results showed that 20 μg/mL SF reduced the lipid binding rate of SZ95 cells by 26.4%; the levels of NO, TNF-α, IL-6 and ROS in RAW 264.7 cells induced by lipolyaccharide (LPS) were decreased by 52.3, 57.5, 56.2 and 35.9%, respectively; The MIC of SF to Propionibacterium acnes, Malassezia furfur and Staphylococcus aureus were 3.125, 12 .5 and 6.25 g/L, respectively, by double dilution method. Through the human efficacy evaluation, the facial acne situation was significantly improved.