玉米醇溶蛋白/鱼腥草黄酮复合纳米颗粒的制备及释放特性

Preparation and release characteristics of zein and flavonoids of Houttuynia cordata composite nanoparticles

摘要:

为提高鱼腥草黄酮(The flavonoids of Houttuynia cordata,HCF)的稳定性,本研究制备了玉米醇溶蛋白/鱼腥草黄酮复合纳米颗粒(Zein/HCF composite nanoparticles,NZH)并对其进行表征,探究zein、HCF不同质量比对NZH的影响。结果表明,HCF与zein之间存在氢键、疏水及静电相互作用；加入HCF后,NZH熔融温度增加,热稳定性提高。随HCF的增加NZH平均粒径、PDI先减小后增大,zeta电位绝对值先增大后减小。其中,NZH-3(zein:HCF为20:3)的平均粒径、PDI值最小、粒径分布峰最窄、zeta电位绝对值最大,微观结构呈均匀分散的圆球状。HCF的加入使复合纳米颗粒具有较高的DPPH、ABTS自由基清除能力。与游离HCF相比,NZH-3具有较好的热处理保护能力。在模拟消化过程中,NZH-3经胃液消化120 min后HCF释放率仅为22.31%,在肠液消化中出现突释现象,胃肠液消化240 min,HCF释放率达到75.66%。NZH-3在模拟胃肠液中的释放趋势均符合Ritger-Peppas模型,但释放机制不同,胃液中属非Fick扩散机制,肠液中属Fick扩散机制。

Abstract:

The aim of this study was to prepare and characterize zein and flavonoids of Houttuynia cordata (HCF) composite nanoparticles (NZH) and investigate the effects of different mass ratios of zein and HCF on the composite nanoparticles. The results showed that hydrogen bonding, hydrophobic and electrostatic interactions existed between HCF and zein, the melting temperature of NZH increased, and the thermal stability improved after the addition of HCF. With the increase of HCF, the average particle size and PDI of NZH first decreased and then increased, and the absolute value of zeta potential and encapsulation first increased and then decreased. Among them, NZH-3 (zein:HCF was 20:3) was homogeneous and well dispersed round spherical shape, the average particle size, the smallest PDI value, the narrowest particle size distribution peak, and the absolute value of zeta potential was the largest. In addition, the addition of HCF made the composite nanoparticles have higher DPPH. and ABTS scavenging ability. Compared with free HCF, NZH-3 had better heat treatment protection ability and improved the heating stability of HCF During the simulated gastrointestinal digestion, the HCF release rate of NZH-3 was only 22.31% after 120 min of digestion by SGF, and the sudden release phenomenon occurred in SIF digestion, and the HCF release rate reached 75.66% at 240 min of digestion by gastrointestinal fluid. NZH-3 release trends in the simulated gastrointestinal fluid were all consistent with the Ritger-Peppas model, but the release mechanisms were different, with a non-Fick diffusion mechanism in SGF and a Fick diffusion mechanism in SIF.