生物基增塑剂己酸异山梨醇二酯的一步酶法合成

One-step enzymatic synthesis of bio-based plasticizer isosorbide hexanoate diester

摘要:

以固定化脂肪酶Novozym 435为催化剂、甲苯为溶剂，加入4?分子筛，催化异山梨醇与正己酸酯化反应制备异山梨醇二己酸酯（SDH），通过单因素实验选择四个因素（反应时间、反应温度、酶添加量、4?分子筛用量）进行响应面分析。结果表明，甲苯10 mL，n(异山梨醇)=0.03 mol，n(异山梨醇)∶n(正己酸)=1∶6时，在反应时间43 h、反应温度40 ℃、固定化脂肪酶Novozym 435 添加量0.1 g、4?分子筛用量为2 g的最优条件下，异山梨醇二己酸酯的产率93.77%。

Abstract:

The immobilized lipase Novozym 435 was used as catalyst, toluene was used as solvent, and 4? molec-ular sieve was added to catalyze the esterification reaction of isosorbide and hexanoic acid to prepare isosorbide dihexanoate (SDH). Four factors (reaction time, reaction temperature, enzyme addition amount, 4? molecular sieve dosage) were selected by single factor experiment for response surface analysis. The results showed that the yield of isosorbide dihexanoate was 93.77 % under the optimal conditions of toluene 10 mL, n ( isosorbide ) = 0.03 mol, n ( isosorbide ) ∶ n ( caproic acid ) = 1 ∶ 6, reaction time 43 h, reaction temperature 40 °C, immobilized lipase Novozym 435 0.1 g and 4? molecu-lar sieve 2 g.