(返修)竹节参多糖通过PI3K/AKT/NF-κB信号通路对ALI大鼠的影响

Effects of bamboo ginseng polysaccharide on ALI rats via PI3K/AKT/NF-κB signaling pathway

摘要:

采用四氯化碳(carbontetrachloride,CCl₄)诱导的急性肝损伤(acute liver injury,ALI)大鼠模型,分别以联苯双酯(阳性药物对照)、低、中、高剂量土家药竹节参多糖(Panaxjaponicuspolysaccharide,PSPJ)对大鼠进行给药,通过观察大鼠一般情况及体重变化,采用苏木素-伊红染色法S(HE)评价PSPJ对肝组织病理变化的改善作用；免疫组织化学(IHC)染色检测肝脏组织NF-κB的表达情况；蛋白免疫印迹法(WB)等方法检测PSPJ对ALI大鼠肝PI3K/AKT/NF-κBR信号通路相关蛋白及炎症指标的表达水平。结果显示,模型组的体重、肝重和肝脏指数均显著上升(P<0.05)；模型组出现大量肝组织炎症浸润,用药组肝脏病理变化明显缓解; 与正常组比较,模型组大鼠肝组织p-PI3K蛋 白 表 达 显著下降( P<0.05), P I3K、p-AKT、 p-NF-κB、IL-1β蛋 白 表 达 显著升 高( P<0.05)； 药物组 p-PI3K蛋白表达显著上升(P<0.05),p-AKT、IL-1β蛋 白 表 达 显著下降( P<0.05)。PSPJ可通过PI3K/AKT/NF-κB通路信号中的相关关键蛋白表达减轻CCL4诱导的大鼠急性肝损伤。

Abstract:

Carbon tetrachloride (CCl4)-induced acute liver injury (ALI) rat model was used to evaluate the effects of PSPJ on liver histopathological changes by observing the general condition and body weight changes of rats, and the improvement of liver histopathological changes by hematoxylin-eosin staining (HE); the effects of PSPJ on liver histopathological changes were evaluated by observing the general condition and body weight changes of rats, and the improvement of liver histopathological changes by hematoxylin-eosin staining (HE). PSPJ) were administered to rats, and the ameliorative effect of PSPJ on the pathological changes of liver tissue was evaluated by observing the general condition and body weight changes of rats, using hematoxylin-eosin staining (HE); immunohistochemistry (IHC) staining to detect the expression of NF-κB in liver tissues; and protein immunoblotting (WB) and other methods to detect the effect of PSPJ on hepatic PI3K/AKT/ NF-κBR signaling pathway-related proteins and expression levels of inflammatory indicators. The results showed that the body weight, liver weight and liver index of the model group increased significantly (P<0.05); a large amount of inflammatory infiltration of liver tissue appeared in the model group, and the pathological changes of the liver in the drug group were obviously alleviated; compared with the normal group, p-PI3K protein expression in the liver tissue of the model group decreased significantly (P<0.05), and the expression of P I3K, p-AKT, p-NF-κB and IL-1β protein increased significantly (P<0.05); in the drug group, the expression of p-PI3K protein increased significantly (P<0.05), and that of p-AKT and IL-1β decreased significantly (P<0.05). In the drug group, p-PI3K protein expression was significantly increased (P<0.05), and p-AKT and IL-1β protein expression was significantly decreased (P<0.05). pSPJ could alleviate CCL4-induced acute liver injury through the expression of key proteins in the signaling of PI3K/AKT/NF-κB pathway in rats.