光热光动力协同抗菌碳点的制备、表征及性能

Preparation, characterization and properties of photothermal/ photodynamic synergistic antibacterial carbon dots

摘要:

以烯丙基三甲基氯化铵为碳源,与氯化铜混合溶解于水中,采用一步水热法合成了具有光热光动力协同抗菌性能的新型铜离子掺杂碳点(CuQACDs)。通过透射扫描电镜、红外光谱、XPS、Zeta电位、Raman光谱、紫外光谱对碳点进行了结构表征。结果表明,CuQACDs为具有良好分散性的球状纳米颗粒,平均粒径约为4.9 nm,ζ电位为+8.17 mV；在1.5 W/cm2 808 nm单一激光照射下,光热转换效率为21.7%,同时可在细菌细胞内显著生成ROS。选用革兰氏阴性菌大肠杆菌(E.coli)及革兰氏阳性菌金黄色葡萄球菌(S.aureus)评价CuQACDs的抗菌性能,CuQACDs对E.coli和S.aureus均有显著抑制效果,在1.5 mg/mL浓度下 用1.5 W/cm2 808 nm激光照射10min后,E.coli的细胞存活率为1.67%,S.aureus的细胞存活率为1.06%；SEM结果表明CuQACDs经激光照射后,细菌表面皱缩、破裂,从而导致细菌死亡。本研究为杀灭致病菌和修复感染伤口提供了一种高效的碳纳米抗菌材料和多模态抗菌方式。

Abstract:

A new type of copper-dopped carbon dots (CuQACDs) with photothermal/ photodynamic synergistic antibacterial ability was prepared via a one-step hydrothermal method using the aqueous solution of CuCl2 and allyl trimethyl ammonium chloride. The CuQACDs was characterized by TEM, FTIR, XPS, Raman, and UV. The obtained CuQACDs were well-dispersed spherical nanoparticles, with the average particle size of 4.9 nm and the zeta potential of +8.17 mV. CuQACDs had a photothermal conversion efficiency of 21.7% and could produce ROS in bacterial cells while being irradiated by a single laser of 808 nm at 1.5 W/cm2. CuQACDs expressed excellent antibacterial capability to E. coli. And S. aureus. The cell viability of E.coli and S.aureus was1.67% and1.06%, respectively after irradiation for 10 min with 808n m laser at1.5 W/cm2. SEM images showed that the surface of bacteria crumpled and ruptured after laser irradiation, leading to bacterial death. This study supplies a highly efficient nanocarbon antibacterial material and multimodal antibacterial method for killing pathogenic bacteria and healing the wounds.