甜玉米芯多糖铁配合物的工艺优化及体外活性

Process optimization and in vitro activity of sweet corn core polysaccharide iron complexes

摘要:

甜玉米芯多糖（SCP80）经三氯化铁共热合成甜玉米芯多糖铁配合物（SCP80—Fe），在单因素实验基础上，采用响应面法优化SCP80—Fe制备工艺。通过SEM，XRD，TG，粒径分布，UV-Vis和FTIR对SCP80—Fe进行结构表征，并进行体外抗氧化及降糖活性的研究。结果表明SCP80—Fe最佳合成工艺为m(SCP80):m(柠檬酸三钠)=3.63:1，pH值为8.5，反应温度为75 ℃，反应时间为64.6 min，此时SCP80—Fe中铁含量（以SCP80—Fe的质量浓度为基准）为27.89±0.35%。SCP80—Fe中的铁是以β—FeOOH铁核的形式与SCP80缔合，相较于SCP80，SCP80—Fe表面光滑，粒径增大，具有更好的热稳定性。体外抗氧化结果显示，SCP80—Fe具有更好的清除羟基自由基能力和还原能力。体外降糖实验显示，SCP80—Fe对α-淀粉酶抑制率的IC50为1.20±0.11 mg/mL，对α-葡萄糖苷酶抑制率的IC50为0.92±0.07 mg/mL，其体外降糖活性明显优于SCP80。

Abstract:

Sweet corn core polysaccharide (SCP80) was co-heated with ferric chloride to synthesize sweet corn core polysaccharide iron complex (SCP80—Fe), and the preparation process of SCP80—Fe was optimized by response surface method on the basis of single-factor experiments. SCP80—Fe was structurally characterized by SEM, XRD, TG, particle size distribution, UV-Vis, and FTIR. Finally, the antioxidant and hypoglycemic activities in vitro were studied. The results showed that the optimal synthesis process of SCP80—Fe was m(SCP80):m(trisodium citrate)=3.63:1, pH value of 8.5, reaction temperature of 75 °C, reaction time of 64.6 min, and the iron content of SCP80—Fe was 27.89±0.35%. The iron in SCP80—Fe is associated with SCP80 in the form of a β—FeOOH iron core, which has a smooth surface, increased particle size and better thermal stability than SCP80. The results of in vitro antioxidant experiments showed that SCP 80—Fe has better hydroxyl radical scavenging and reducing capabilities. The results of in vitro hypoglycemic experiments showed that the IC50s of SCP80—Fe for the inhibition rates of α-amylase and α-glucosidase were 1.20±0.11 mg/mL and 0.92±0.07 mg/mL, respectively, which had better in vitro hypoglycemic activity than SCP80.