超声辅助深共熔溶剂提取栀子黄酮及其对HepG2细胞的降血糖作用

Optimization of ultrasonic-assisted deep eutectic solvent extraction of gardenia flavone and its hypoglycemic effect on HepG2 cells

摘要:

目的:探究超声辅助深共熔溶剂法(deep eutectic solvent,DES)提取栀子黄酮的工艺流程,并研究黄酮对HepG2细胞的降糖作用。方法:采用单因素、正交法优化超声辅助深共熔溶剂提取栀子黄酮的最佳工艺,并通过测定其对HepG2细胞的降糖作用探讨了栀子黄酮的体外降血糖能力。结果:栀子黄酮提取的最佳工艺为:选择的深共熔溶剂为氯化胆碱:1,2-丙二醇的摩尔比为1:3,超声时间40 min、超声温度60℃、含水量20%、料液比为1:33(g/mL)。此外,细胞体外降血糖活性研究表明栀子黄酮具有良好的降血糖活性。结论:本研究提供了一种绿色高效的从栀子中提取黄酮的方法,且该黄酮在一定程度上具有降血糖活性,旨在为栀子产业化奠定基础。

Abstract:

Objective: To study the extraction process of flavonoids from gardenia gardenia by ultrasonic-assisted deep eutectic solvent (DES), and to study the hypoglycemic effect of flavonoids on HepG2 cells. Methods: Single factor and orthogonal method were used to optimize the extraction process of gardenia flavone with ultrasonic-assisted deep eutectic solvent. The hypoglycemic effect of gardenia flavone on HepG2 cells in vitro was investigated. Results: The optimal extraction process of flavonoids from gardenia gardenia was as follows: the deep eutectic solvent was choline chloride, the molar ratio of 1, 2-propylene glycol was 1:3, the ultrasonic time was 40 min, the ultrasonic temperature was 60℃, the water content was 20%, the ratio of solid to liquid was 1:33 (g/mL). In addition, in vitro hypoglycemic activity study showed that gardenia flavone has good hypoglycemic activity. Conclusion: This study provides a green and efficient method to extract flavone from gardenia gardenia, and the flavonoid has hypoglycemic activity to a certain extent, which aims to lay a foundation for the industrialization of gardenia gardenia.