接力识别Al3+及F-的偶氮类荧光探针合成与应用

Synthesis and application of azo-based fluorescent probe for relay recognition of Al3+ and F-

摘要:

基于6-氨基间甲酚和1-萘酚成功合成并表征了一种新型的偶氮类荧光探针(NA-M)。在MeOH/HEPES(9/1,v/v,pH=7.4)溶液中,于590 nm处NA-M与Al₃₊^{发生显著的荧光增强}作用,并对Al₃₊^{表现出高度的特异性、}抗干扰性和良好的灵敏度(8.4×10_-7 ^M)。通过Job"s plot实验确定了Al₃₊^与探针NA-M之间的配位比为1:2。与此同时,络合物NA-M+Al₃₊^对F_-^发生荧光淬灭作用,显示出高选择性和良好的抗干扰能力,络合物NA-M+Al₃₊^对F_-^{的检测限度可达到}9.5×10_-7 ^M。最后,探针NA-M及其复合物NA-M+Al₃₊^也分别成功应用于实际样品中的Al₃₊^及F_-^的检测。

Abstract:

A noval azo-based fluorescent probe (NA-M), derived from 6-amino-m-cresol and 1-naphthol, was successfully synthesized and characterized. NA-M had a significant enhancement of fluorescence with Al₃₊ ^{at 590 nm in} MeOH/HEPES (9/1, v/v, pH=7.4) solution, and displayed high specificity, strong anti-interference and good sensitivity (8.4×10_-7 ^M). The 1:2 binding stoichiometry between Al₃₊ ^and probe had been established from Job’s plot. Meanwhile, the complex NA-M+Al₃₊ showed high selectivity and good anti-interference ability to F_- by quenching the fluorescence, and the detection limit of complex NA-M+Al₃₊ ^to F_- ^{could reach} to 9.5×10_-7 M. Eventually, probe NA-M and its complex NA-M+Al₃₊ had also been successfully applied to detect Al₃₊ ^and F_- ⁱⁿ actual samples, respectively.